如果基因治疗能够彻底根除乙肝，大家能够接受多少？

StephenW

lifflefield 发表于 2011-9-26 14:42
1.请google  tet on and tet off 很多回答的。2.只有一次侵染，对的。 - how?
－－－－－－－－－－－－ ...

2. You are saying that you can engineer the AAV virus not to replicate (no expression of its viral genes, therefor no viral proteins to re-constitute a new AAV virus?), yet it will only allow the expression of the K-gene to produce your k-protein? If your rAAV cannot replicate, how will you produce  the massive amount of rAAV for injection into the human body?
3.You mean expression of the k-gene to produce k-protein, no problem. This is what Gene therapy is all about.
4. What other gene expression regulation systems are you talking about?

Finally, I know this is pedantic, what about hbv mutants that can escape your engineered "immune control" (unless it is so powerful that it can knock out all the cccDNA)?

走遍四方

回复 StephenW 的帖子

Finally, I know this is pedantic, what about hbv mutants that can escape your engineered "immune control" (unless it is so powerful that it can knock out all the cccDNA)?
--------------k 因子可能target 的是各个hbv 中共有的东西，mutant的hbv也只是hbv中某些点位

StephenW

走遍四方 发表于 2011-9-26 16:27
回复 StephenW 的帖子

Finally, I know this is pedantic, what about hbv mutants that can escape your  ...

I know I am just being difficult, but I guess k-factor is based on APBEC3G, and HIV has evolved a defence mechanism against it. Likewise, many viruses have evolved mechanism to reduce the antiviral effects of Interferon.


APOBEC3G (apoplipoprotein B mRNA-editing, enzyme-catalytic, polypeptide-like 3G) is a human enzyme encoded by the APOBEC3G gene that belongs to the APOBEC superfamily of proteins.[1] This family of proteins has been suggested to play an important role in innate anti-viral immunity.[2] APOBEC3G is a cytadine deaminase enzyme and is therefore known as DNA dC->dU-editing enzyme APOBEC-3G and APOBEC-related cytidine deaminase (ARCD).[1]
APOBEC3G exerts innate antiretroviral immune activity against retroviruses, most notably HIV, by interfering with proper replication. However, lentiviruses such as HIV have evolved the Viral infectivity factor (Vif) protein in order to counteract this effect. Vif interacts with APOBEC3G and triggers the ubiquitination and degradation of APOBEC3G via the proteasomal pathway.[3]

走遍四方

回复 StephenW 的帖子

Not difficult, very precise,.
不难处，很严谨，
All you concerns must be cleared when Go-live to practice.

蔡小楠

好像是中国人的东西哦！！！可靠吗？

yh0046

回复 lifflefield 的帖子

楼主，我非常支持你的研究，我是个生物学教师，我愿意接受基因治疗！只希望它能早日成功，早日让广大患者脱离苦海！

yh0046

我能接收基因治疗，我是中学教师，也可起到宣传作用，希望该技术早日成功！

cshbv

回复 lifflefield 的帖子

如果基因治疗能够彻底根除乙肝，大家能够接受多少？-- 你的题目是什么意思？能够接受多少钱吗？还是接受多少k因子注射量？
相信如果你们的技术可行的话，找合作伙伴，或者找临床实验对象，是不成问题的。
国内有家RNAi的顶尖公司Biomics也在研究HBV的RNAi方案。请问你对他们的方法有所了解吗？或者有所比较？如果能比他们强，那估计申请国家基金项目，或者跟国内的机构联合申请都应该是没问题的。
另外国内有困难的话，可以去别的国家，世界上不是只有中国有乙肝的问题。相信很多国家会比中国更加透明和注重知识产权保护。

brucexm

to ifflefield，对于大家能够接受多少这问题，个人觉得，如果您提供的方法要适用临床，首先还是考量与医院单位的合作，然后基于医院单位的信用，国内患者应该会有一个比较好的接受心理预期。


附上一张HBV复制机理图解，应该能有助于各位对ifflefield某些说明的理解。

HBV复制图




ifflefield提供一个很好的应用方向，大家努力提问题

foto2002

The U.S. government and FDA are selfish . The U.S. government declared they want to save the humanright, but they  neglecting 130 million Chinese people‘s suffering . Don’t forget us! We also are the human. We like the blue sky, we also want to play in the sunshine , we love our life.Why you to authorized the K-gene for the HIV ?Why not go with the HBV?Don‘t criticized our country is no humanright. I hate the hypocritical guys!