基于高通量长读长测序的肝癌细胞乙肝病毒基因组整合模型

StephenW

基于高通量长读长测序的肝癌细胞乙肝病毒基因组整合模型
未央李 1 未未 2 飞侯 2 韩世旭 3 崔小芳 4
隶属关系
隶属关系

    1
    济宁医科大学，山东济宁272067；济宁医科大学山东省出生缺陷研究与转化协同创新中心，山东济宁272067电子地址：[email protected]。
    2
    济宁医科大学，山东济宁 272067
    3
    中山大学肿瘤中心鼻咽癌科，广东 广州 510275
    4
    济宁医科大学，山东济宁 272067电子地址：[email protected]。

    PMID：34843903 DOI：10.1016/j.ygeno.2021.11.025

抽象的

HBV的整合和功能已逐渐扩大。然而，HBV整合的确切模式仍不清楚。在我们的研究中，高通量长读长测序与生物信息学相结合，研究了肝细胞癌 (HCC) 细胞中 HBV 整合的完整模式。结果表明：1）HBV整合事件的HBV插入序列占HBV总序列的49.5%。 2）长度为0-1 kbp的短插入片段占50%，长插入片段（>3 kbp）占HBV插入事件的25%。 3)不同区域内形成的断点存在不同的HBV插入长度。 4）HBV整合事件的发生伴随着更频繁的结构变异。 5）此外，基于完整的HBV插入序列确认了多个HBV整合模式。我们的研究不仅阐明了各种完美的HBV整合模型，而且确定了HBV整合的多个具体特征。

关键词：染色体易位； HBV整合； HBV插入序列；肝细胞癌；长读长测序；完善的融合模式。

版权所有 © 2021。Elsevier Inc. 出版。

StephenW

The integration model of hepatitis B virus genome in hepatocellular carcinoma cells based on high-throughput long-read sequencing
Weiyang Li  1 , Wei Wei  2 , Fei Hou  2 , Hanshi Xu  3 , Xiaofang Cui  4
Affiliations
Affiliations

    1
    Jining Medical University, Jining, Shandong 272067, China; Collaborative Innovation Center for Birth Defect Research and Transformation of Shandong Province, Jining Medical University, Jining, Shandong 272067, China. Electronic address: [email protected].
    2
    Jining Medical University, Jining, Shandong 272067, China.
    3
    Department of Nasopharyngeal Carcinoma, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong 510275, China.
    4
    Jining Medical University, Jining, Shandong 272067, China. Electronic address: [email protected].

    PMID: 34843903 DOI: 10.1016/j.ygeno.2021.11.025

Abstract

HBV integration and function has gradually been expanding. However, the exact mode of HBV integration remains unclear. In our research, the high-throughput long-read sequencing was combined with bioinformatics to study the complete mode of HBV integration in hepatocellular carcinoma (HCC) cells. The results demonstrated that: 1) The HBV insertion sequences of HBV integration events accounted for 49.5% of the total HBV sequences. 2) Short insertion segments with the length of 0-1 kbp accounted for 50% and the long insertion segments (>3 kbp) accounted for 25% of HBV insertion events. 3)There were different HBV insertion length in the breakpoints formed within different regions. 4) The occurrence of HBV integration events was accompanied by more frequent structural variations. 5)Furthermore, multiple HBV integration patterns were confirmed based on complete HBV insertion sequences. Our research not only clarified a variety of perfect HBV integration models but also determined multiple specific features of HBV integration.

Keywords: Chromosome translocation; HBV Integration; HBV insertion sequence; HCC; Long-reads sequencing; Perfect integration mode.

Copyright © 2021. Published by Elsevier Inc.