The integration model of hepatitis B virus genome in hepatocellular carcinoma cells based on high-throughput long-read sequencing
Weiyang Li 1 , Wei Wei 2 , Fei Hou 2 , Hanshi Xu 3 , Xiaofang Cui 4
Affiliations
Affiliations
1
Jining Medical University, Jining, Shandong 272067, China; Collaborative Innovation Center for Birth Defect Research and Transformation of Shandong Province, Jining Medical University, Jining, Shandong 272067, China. Electronic address: [email protected].
2
Jining Medical University, Jining, Shandong 272067, China.
3
Department of Nasopharyngeal Carcinoma, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong 510275, China.
4
Jining Medical University, Jining, Shandong 272067, China. Electronic address: [email protected].
PMID: 34843903 DOI: 10.1016/j.ygeno.2021.11.025
Abstract
HBV integration and function has gradually been expanding. However, the exact mode of HBV integration remains unclear. In our research, the high-throughput long-read sequencing was combined with bioinformatics to study the complete mode of HBV integration in hepatocellular carcinoma (HCC) cells. The results demonstrated that: 1) The HBV insertion sequences of HBV integration events accounted for 49.5% of the total HBV sequences. 2) Short insertion segments with the length of 0-1 kbp accounted for 50% and the long insertion segments (>3 kbp) accounted for 25% of HBV insertion events. 3)There were different HBV insertion length in the breakpoints formed within different regions. 4) The occurrence of HBV integration events was accompanied by more frequent structural variations. 5)Furthermore, multiple HBV integration patterns were confirmed based on complete HBV insertion sequences. Our research not only clarified a variety of perfect HBV integration models but also determined multiple specific features of HBV integration.