真皮内免疫与自体白细胞介导强化慢性乙型肝炎的抗病毒治

StephenW

INTENSIFICATION OF ANTIVIRAL THERAPY OF CHRONIC HEPATITIS B BY MEANS OF INTRADERMAL IMMUNIZATION WITH AUTOLEUKOCYTES
Alexandr N Zinchuk  1 , Olga A Golubovska  2 , Borys A Herasun  1 , Andrii M Zadorozhnyi  1 , Oleksandr B Herasun  1
Affiliations
Affiliations

    1
    DANYLO HALYTSKY LVIV NATIONAL MEDICAL UNIVERSITY, LVIV, UKRAINE.
    2
    BOGOMOLETS NATIONAL MEDICAL UNIVERSITY, KYIV, UKRAINE.

    PMID: 33310939

Abstract

Objective: The aim: Was a trial of intradermal immunization with native autoleukocytes as a curative vaccine.

Patients and methods: Materials and methods: Thus, 3-shot series vaccination by means of intradermal injection of autoleukocytes with 30 - 40 days interval was conducted for patients who, in spite of continuous (at least 2 years) therapy with nucleotide analogue, experienced HBV DNA reproduction. For this procedure, 80 - 100 ml of a patient's heparinized venous blood was being precipitated at the temperature 37оС for 120 - 140 minutes, after blood plasma was being centrifuged at 450g for 8 minutes. The precipitate was resuspended in 1 - 1.5 ml of blood serum and injected intradermally in the region of the back.

Results: Results: Viral load decreased in all patients even after single immunization; it was possible to achieve a negative result by ultrasensitive PCR method in 23.33% of patients in the group of patients who did not respond adequately to antiviral therapy.

Conclusion: Conclusions: In patients with chronic hepatitis B, intradermal immunization with autoleukocytes has a significant impact on intensity of virus replication. It is confirmed by a considerable reduction of DNA HBV amount in patients, in whom antiviral therapy was stopped before immunization.

Keywords: autoleukocyte vaccination; chronic hepatitis B; therapy with nucleotide analogue.

StephenW

真皮内免疫与自体白细胞介导强化慢性乙型肝炎的抗病毒治疗
Alexandr N Zinchuk 1，Olga A Golubovska 2，Borys A Herasun 1，Andrii M Zadorozhnyi 1，Oleksandr B Herasun 1
隶属关系
隶属关系

    1个
    DANYLO HALYTSKY LVIV国家医科大学，乌克兰LVIV。
    2
    乌克兰基辅，波哥大国立医科大学。

    PMID：33310939

抽象

目的：目的：进行以天然自体白细胞为治愈性疫苗的皮内免疫试验。

患者和方法：材料和方法：因此，尽管连续（至少2年）接受核苷酸类似物治疗，但仍经历了30到40天间隔的皮内注射自体白细胞的3针系列疫苗接种HBV DNA复制。在此过程中，将血浆以450g离心8分钟后，将80-100 ml患者的肝素化静脉血在37℃下沉淀120-140分钟。将沉淀物重悬于1-1.5ml的血清中，并在背部区域内皮内注射。

结果：结果：即使单次免疫后，所有患者的病毒载量均下降；在对抗病毒治疗没有充分反应的患者组中，通过超敏PCR方法可能有23.33％的患者获得阴性结果。

结论：结论：在慢性乙型肝炎患者中，自体白细胞的皮内免疫对病毒复制强度有重要影响。通过在免疫前停止抗病毒治疗的患者中DNA HBV量的显着降低，可以证实这一点。

关键词：自身白细胞疫苗；慢性乙型肝炎核苷酸类似物的疗法。