INTENSIFICATION OF ANTIVIRAL THERAPY OF CHRONIC HEPATITIS B BY MEANS OF INTRADERMAL IMMUNIZATION WITH AUTOLEUKOCYTES
Alexandr N Zinchuk 1 , Olga A Golubovska 2 , Borys A Herasun 1 , Andrii M Zadorozhnyi 1 , Oleksandr B Herasun 1
Affiliations
Affiliations
1
DANYLO HALYTSKY LVIV NATIONAL MEDICAL UNIVERSITY, LVIV, UKRAINE.
2
BOGOMOLETS NATIONAL MEDICAL UNIVERSITY, KYIV, UKRAINE.
PMID: 33310939
Abstract
Objective: The aim: Was a trial of intradermal immunization with native autoleukocytes as a curative vaccine.
Patients and methods: Materials and methods: Thus, 3-shot series vaccination by means of intradermal injection of autoleukocytes with 30 - 40 days interval was conducted for patients who, in spite of continuous (at least 2 years) therapy with nucleotide analogue, experienced HBV DNA reproduction. For this procedure, 80 - 100 ml of a patient's heparinized venous blood was being precipitated at the temperature 37оС for 120 - 140 minutes, after blood plasma was being centrifuged at 450g for 8 minutes. The precipitate was resuspended in 1 - 1.5 ml of blood serum and injected intradermally in the region of the back.
Results: Results: Viral load decreased in all patients even after single immunization; it was possible to achieve a negative result by ultrasensitive PCR method in 23.33% of patients in the group of patients who did not respond adequately to antiviral therapy.
Conclusion: Conclusions: In patients with chronic hepatitis B, intradermal immunization with autoleukocytes has a significant impact on intensity of virus replication. It is confirmed by a considerable reduction of DNA HBV amount in patients, in whom antiviral therapy was stopped before immunization.