具有对各种病毒基因型和临床突变体的乙肝病毒表面抗原具

StephenW

PLoS One

. 2020 Aug 13;15(8):e0236704.
doi: 10.1371/journal.pone.0236704. eCollection 2020.
A recombinant human immunoglobulin with coherent avidity to hepatitis B virus surface antigens of various viral genotypes and clinical mutants
Gi Uk Jeong  1 , Byung-Yoon Ahn  1 , Jaesung Jung  2 , Hyunjin Kim  2 , Tae-Hee Kim  2 , Woohyun Kim  2 , Ara Lee  2 , Kyuhyun Lee  3 , Jung-Hwan Kim  2
Affiliations

    PMID: 32790777 DOI: 10.1371/journal.pone.0236704

Abstract

The hepatitis B virus (HBV) envelope is composed of a lipid bilayer and three glycoproteins, referred to as the large (L), middle (M), and small (S) hepatitis B virus surface antigens (HBsAg). S protein constitutes the major portion of the viral envelope and an even greater proportion of subviral particles (SVP) that circulate in the blood. Recombinant S proteins are currently used as a preventive vaccine, while plasma fractions isolated from vaccinated people, referred to as hepatitis B immune globulin (HBIG), are used for short-term prophylaxis. Here, we characterized a recombinant human IgG1 type anti-S antibody named Lenvervimab regarding its binding property to a variety of cloned S antigens. Immunochemical data showed an overall consistent avidity of the antibody to S antigens of most viral genotypes distributed worldwide. Further, antibody binding was not affected by the mutations in the antigenic 'a' determinant found in many clinical variants, including the immune escape mutant G145R. In addition, mutations in the S gene sequence that confer drug resistance to the viral polymerase did not interfere with the antibody binding. These results support for a preventive use of the antibody against HBV infection.

StephenW

。 2020年8月13日； 15（8）：e0236704。
doi：10.1371 / journal.pone.0236704。 eCollection 2020。
具有对各种病毒基因型和临床突变体的乙肝病毒表面抗原具有连贯亲和力的重组人免疫球蛋白
Gi Uk Jeong 1，Bing-Yoon Ahn 1，Jaesung Jung 2，Hyunjin Kim 2，Tae-Hee Kim 2，Woohyun Kim 2，Ara Lee 2，Kyuhyun Lee 3，Jung-Hwan 2
隶属关系

    PMID：32790777 DOI：10.1371 / journal.pone.0236704

抽象

乙型肝炎病毒（HBV）包膜由脂质双层和三种糖蛋白组成，称为大（L），中（M）和小（S）乙型肝炎病毒表面抗原（HBsAg）。 S蛋白构成病毒包膜的主要部分，并且在血液中循环传播的亚病毒颗粒（SVP）所占比例甚至更高。重组S蛋白目前用作预防性疫苗，而从接种人群中分离的血浆级分（称为B型肝炎免疫球蛋白（HBIG））则用于短期预防。在这里，我们就其与多种克隆的S抗原的结合特性，对名为Lenvervimab的重组人IgG1型抗S抗体进行了表征。免疫化学数据显示，该抗体对全世界分布的大多数病毒基因型的S抗原具有总体一致的亲和力。此外，抗体结合不受在许多临床变体中发现的抗原性'a'决定簇突变的影响，包括免疫逃避突变体t G145R。此外，赋予病毒对聚合酶抗药性的S基因序列突变不会干扰抗体结合。这些结果支持预防性使用抗HBV感染的抗体。

StephenW

https://journals.plos.org/ploson ... ournal.pone.0236704