血浆环状RNA小组诊断乙型肝炎病毒相关的肝细胞癌：一项大

StephenW

Int J Cancer. 2019 Aug 28. doi: 10.1002/ijc.32647. [Epub ahead of print]
Plasma Circular RNA Panel to Diagnose Hepatitis B Virus-Related Hepatocellular Carcinoma: A Large-Scale, Multicenter Study.
Yu J1,2,3, Ding WB1,2,3, Wang MC1,2,3, Guo XG1,2,3, Xu J4, Xu QG1,2,3, Yang Y1,2,3, Sun SH5,6, Liu JF7, Qin LX8, Liu H1,2,3, Yang F5,6, Zhou WP1,2,3.
Author information

1
    The Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China.
2
    Key Laboratory of Signaling Regulation and Targeting Therapy of Liver Cancer (SMMU)，Ministry of Education, Shanghai 200438, China.
3
    Shanghai Key Laboratory of Hepatobiliary Tumor Biology (EHBH), Shanghai 200438, China.
4
    Department of Laboratory Diagnosis, Changhai Hospital, Second Military Medical University, Shanghai 200438, China.
5
    Department of Medical Genetics, Second Military Medical University, Shanghai 200433, China.
6
    Shanghai Key Laboratory of Cell Engineering (14DZ2272300), People's Republic of China.
7
    Mengchao Hepatobiliary Hospital, Fujian Medical University, Fuzhou, 350025, China.
8
    Department of General Surgery, Huashan Hospital & Cancer Metastasis Institute & Institutes of Biomedical Sciences, Fudan University, Shanghai 200040, China.

Abstract

To explore whether plasma circular RNAs (circRNAs) can diagnose hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC), microarray and qPCR were used to identify plasma circRNAs that were increased in HBV-related HCC patients compared with controls (including healthy controls, chronic hepatitis B and HBV-related liver cirrhosis). A logistic regression model was constructed using a training set (n=313) and then validated using another two independent sets (n=306 and 526, respectively). Area under the receiver operating characteristic curve (AUC) was used to evaluate diagnostic accuracy. We identified a plasma circRNA panel (CircPanel) containing three circRNAs (hsa_circ_0000976, hsa_circ_0007750 and hsa_circ_0139897) that could detect HCC. CircPanel showed a higher accuracy than AFP (alpha-fetoprotein) to distinguish individuals with HCC from controls in all three sets (AUC 0.863 [95% CI 0.819-0.907] vs 0.790 [0.738-0.842], P=0.036 in training set; 0.843 [0.796-0.890] vs 0.747 [0.691-0.804], P=0.011 in validation set 1 and 0.864 [0.830-0.898] vs 0.769 [0.728-0.810], P<0.001 in validation set 2). CircPanel also performed well in detecting Small-HCC (solitary, ≤3cm), AFP-negative HCC and AFP-negative Small-HCC. This article is protected by copyright. All rights reserved.

This article is protected by copyright. All rights reserved.
KEYWORDS:

CircPanel; Circular RNA; Diagnosis; Hepatocellular carcinoma; Plasma

PMID:
    31456215
DOI:
    10.1002/ijc.32647

StephenW

Int J Cancer。 2019年8月28日doi：10.1002 / ijc.32647。 [印刷前的电子版]
血浆环状RNA小组诊断乙型肝炎病毒相关的肝细胞癌：一项大规模，多中心研究。
Yu J1,2,3，Ding WB1,2,3，Wang MC1,2,3，Guo XG1,2,3，Xu J4，Xu QG1,2,3，Yang Y1,2,3，Sun SH5,6， Liu JF7，Qin LX8，Liu H1,2,3，Yang F5,6，Zhou WP1,2,3。
作者信息

1
第二军医大学东方肝胆外科医院肝外科，上海200438
2
2004年上海市教育部信号调控与肝癌靶向治疗重点实验室（SMMU）。
3
上海市肝胆肿瘤生物学重点实验室（EHBH），上海200438
4
第二军医大学长海医院检验科，上海200438
五
第二军医大学医学遗传学系，上海200433
6
中华人民共和国上海市细胞工程重点实验室（14DZ2272300）。
7
福建医科大学孟超肝胆外科医院，福州350025
8
复旦大学华山医院普外科，肿瘤转移研究所，生物医学科学研究所，上海200040

抽象

为了探索血浆环状RNA（circRNA）是否可以诊断乙型肝炎病毒（HBV）相关的肝细胞癌（HCC），微阵列和qPCR用于鉴定与对照组（包括健康对照组）相比，HBV相关HCC患者血浆circRNA增加慢性病和特征曲线（AUC）用于评估诊断的准确性。我们确定了一个血浆circRNA组（CircPanel），其中含有三个可以检测HCC的circRNAs（hsa_circ_0000976，hsa_circ_0007750和hsa_circ_0139897）.CircPanel显示出比AFP更高的准确性（甲胎蛋白）对所有三组患者的HCC患者（AUC 0.863 [95％CI 0.819-0.907] vs 0.790 [0.738-0.842]，训练组P = 0.036; 0.843 [0.796-0.890] vs 0.747 [ 0.691-0.804]，验证组1中P = 0.011，0.864 [0.830-0.898]对0.769 [0.728-0.810]，验证组2中P <0.001。CircPanel在检测小肝癌方面也表现良好（孤立，≤3cm） ），AFP阴性HCC和AFP阴性Smal 1-HCC。本文受版权保护。版权所有。

本文受版权保护。版权所有。
关键词：

CircPanel;环状RNA;诊断;肝细胞癌;等离子体

结论：
31456215
DOI：
10.1002 / ijc.32647