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[其他] 血浆环状RNA小组诊断乙型肝炎病毒相关的肝细胞癌:一项大 [复制链接]

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才高八斗

1
发表于 2019-9-1 20:45 |只看该作者 |倒序浏览 |打印
Int J Cancer. 2019 Aug 28. doi: 10.1002/ijc.32647. [Epub ahead of print]
Plasma Circular RNA Panel to Diagnose Hepatitis B Virus-Related Hepatocellular Carcinoma: A Large-Scale, Multicenter Study.
Yu J1,2,3, Ding WB1,2,3, Wang MC1,2,3, Guo XG1,2,3, Xu J4, Xu QG1,2,3, Yang Y1,2,3, Sun SH5,6, Liu JF7, Qin LX8, Liu H1,2,3, Yang F5,6, Zhou WP1,2,3.
Author information

1
    The Third Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai 200438, China.
2
    Key Laboratory of Signaling Regulation and Targeting Therapy of Liver Cancer (SMMU),Ministry of Education, Shanghai 200438, China.
3
    Shanghai Key Laboratory of Hepatobiliary Tumor Biology (EHBH), Shanghai 200438, China.
4
    Department of Laboratory Diagnosis, Changhai Hospital, Second Military Medical University, Shanghai 200438, China.
5
    Department of Medical Genetics, Second Military Medical University, Shanghai 200433, China.
6
    Shanghai Key Laboratory of Cell Engineering (14DZ2272300), People's Republic of China.
7
    Mengchao Hepatobiliary Hospital, Fujian Medical University, Fuzhou, 350025, China.
8
    Department of General Surgery, Huashan Hospital & Cancer Metastasis Institute & Institutes of Biomedical Sciences, Fudan University, Shanghai 200040, China.

Abstract

To explore whether plasma circular RNAs (circRNAs) can diagnose hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC), microarray and qPCR were used to identify plasma circRNAs that were increased in HBV-related HCC patients compared with controls (including healthy controls, chronic hepatitis B and HBV-related liver cirrhosis). A logistic regression model was constructed using a training set (n=313) and then validated using another two independent sets (n=306 and 526, respectively). Area under the receiver operating characteristic curve (AUC) was used to evaluate diagnostic accuracy. We identified a plasma circRNA panel (CircPanel) containing three circRNAs (hsa_circ_0000976, hsa_circ_0007750 and hsa_circ_0139897) that could detect HCC. CircPanel showed a higher accuracy than AFP (alpha-fetoprotein) to distinguish individuals with HCC from controls in all three sets (AUC 0.863 [95% CI 0.819-0.907] vs 0.790 [0.738-0.842], P=0.036 in training set; 0.843 [0.796-0.890] vs 0.747 [0.691-0.804], P=0.011 in validation set 1 and 0.864 [0.830-0.898] vs 0.769 [0.728-0.810], P<0.001 in validation set 2). CircPanel also performed well in detecting Small-HCC (solitary, ≤3cm), AFP-negative HCC and AFP-negative Small-HCC. This article is protected by copyright. All rights reserved.

This article is protected by copyright. All rights reserved.
KEYWORDS:

CircPanel; Circular RNA; Diagnosis; Hepatocellular carcinoma; Plasma

PMID:
    31456215
DOI:
    10.1002/ijc.32647

Rank: 8Rank: 8

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62111 元 
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30437 
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才高八斗

2
发表于 2019-9-1 20:46 |只看该作者
Int J Cancer。 2019年8月28日doi:10.1002 / ijc.32647。 [印刷前的电子版]
血浆环状RNA小组诊断乙型肝炎病毒相关的肝细胞癌:一项大规模,多中心研究。
Yu J1,2,3,Ding WB1,2,3,Wang MC1,2,3,Guo XG1,2,3,Xu J4,Xu QG1,2,3,Yang Y1,2,3,Sun SH5,6, Liu JF7,Qin LX8,Liu H1,2,3,Yang F5,6,Zhou WP1,2,3。
作者信息

1
第二军医大学东方肝胆外科医院肝外科,上海200438
2
2004年上海市教育部信号调控与肝癌靶向治疗重点实验室(SMMU)。
3
上海市肝胆肿瘤生物学重点实验室(EHBH),上海200438
4
第二军医大学长海医院检验科,上海200438

第二军医大学医学遗传学系,上海200433
6
中华人民共和国上海市细胞工程重点实验室(14DZ2272300)。
7
福建医科大学孟超肝胆外科医院,福州350025
8
复旦大学华山医院普外科,肿瘤转移研究所,生物医学科学研究所,上海200040

抽象

为了探索血浆环状RNA(circRNA)是否可以诊断乙型肝炎病毒(HBV)相关的肝细胞癌(HCC),微阵列和qPCR用于鉴定与对照组(包括健康对照组)相比,HBV相关HCC患者血浆circRNA增加慢性病和特征曲线(AUC)用于评估诊断的准确性。我们确定了一个血浆circRNA组(CircPanel),其中含有三个可以检测HCC的circRNAs(hsa_circ_0000976,hsa_circ_0007750和hsa_circ_0139897).CircPanel显示出比AFP更高的准确性(甲胎蛋白)对所有三组患者的HCC患者(AUC 0.863 [95%CI 0.819-0.907] vs 0.790 [0.738-0.842],训练组P = 0.036; 0.843 [0.796-0.890] vs 0.747 [ 0.691-0.804],验证组1中P = 0.011,0.864 [0.830-0.898]对0.769 [0.728-0.810],验证组2中P <0.001。CircPanel在检测小肝癌方面也表现良好(孤立,≤3cm) ),AFP阴性HCC和AFP阴性Smal 1-HCC。本文受版权保护。版权所有。

本文受版权保护。版权所有。
关键词:

CircPanel;环状RNA;诊断;肝细胞癌;等离子体

结论:
31456215
DOI:
10.1002 / ijc.32647
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