成簇的规律间隔短回文重复序列及其相关蛋白9系统是近年来

StephenW

Zhonghua Gan Zang Bing Za Zhi. 2018 Nov 20;26(11):860-864. doi: 10.3760/cma.j.issn.1007-3418.2018.11.012.
[Application of clustered regularly interspaced short palindromic repeats- associated protein 9 gene editing technology for treatment of HBV infection].
[Article in Chinese; Abstract available in Chinese from the publisher]
Wang YD1, Liang QF, Li ZY, Zhao CY.
Author information

1
    Department of Infectious Diseases, the Third Affiliated Hospital of Hebei Medical University, Shijiazhuang 050035, China.

Abstractin English, Chinese

缺乏有效根除乙型肝炎病毒（HBV）的药物及技术手段是限制HBV感染无法治愈的瓶颈。成簇的规律间隔短回文重复序列及其相关蛋白9系统是近年来新兴的对特定基因位点进行编辑的技术，可以特异性靶向HBV共价闭合环状DNA，有效抑制HBV DNA复制、调控HBV功能蛋白表达，有望成为彻底根除HBV的强有力基因治疗工具。如何利用成簇的规律间隔短回文重复序列及其相关蛋白9系统实现对HBV基因组高效靶向修饰，从而达到根治HBV感染的目的已经成为当前国内外学者关注的焦点。现结合国内外最新研究成果，重点总结成簇的规律间隔短回文重复序列及其相关蛋白9基因编辑技术在抗HBV感染治疗中应用的最新进展，阐述其作为彻底根治HBV感染方案的潜在可能和挑战。.
KEYWORDS:

Clustered regularly interspaced short palindromic repeats/CRISPR-associated nuclease 9; Covalently closed circle DNA; Gene editing; Hepatitis B virus

PMID:
    30616324