乙型肝炎病毒感染肝细胞中细胞外囊泡的免疫功能。

StephenW

PLoS One. 2018 Dec 31;13(12):e0205886. doi: 10.1371/journal.pone.0205886. eCollection 2018.
The immunological function of extracellular vesicles in hepatitis B virus-infected hepatocytes.
Kakizaki M1,2, Yamamoto Y1, Yabuta S1,3, Kurosaki N3, Kagawa T2, Kotani A1,3.
Author information

1
    Division of Hematological Malignancy, Institute of Medical Sciences, Tokai University, Isehara, Japan.
2
    Division of Gastroenterology and Hepatology, Department of Internal Medicine, Tokai University School of Medicine, Isehara, Japan.
3
    Department of Hematology and Oncology, Tokai University School of Medicine, Isehara, Japan.

Abstract

Hepatitis B virus (HBV) generates large amounts of complete and incomplete viral particles. Except for the virion, which acts as infectious particles, the function of those particles remains elusive. Extracellular vesicles (EVs) have been revealed to have biological functions. The EVs which size are less than 100 nm in diameter, were collected from HBV infected-patients. These vesicles contain, complete and incomplete virions, and exosomes, which have been recently shown to be critical as intercellular communicators. Here, the effects of the exosome, the complete, and the incomplete particles on the target cells were investigated. These particles are endocytosed by monocyte/macrophages and function primarily to upregulate PD-L1. The functions and composition of the EVs were affected by nucleotide reverse transcriptase inhibitors (NRTIs), suggesting that the EVs are involved in the pathogenesis of HBV hepatitis and clinical course of those patients treated by NRTIs.

PMID:
    30596665
DOI:
    10.1371/journal.pone.0205886

StephenW

PLoS One。 2018年12月31日; 13（12）：e0205886。 doi：10.1371 / journal.pone.0205886。 eCollection 2018。
乙型肝炎病毒感染肝细胞中细胞外囊泡的免疫功能。
Kakizaki M1,2，Yamamoto Y1，Yabuta S1,3，Kurosaki N3，Kagawa T2，Kotani A1,3。
作者信息

1
    日本伊势原东海大学医学科学研究所血液系恶性科
2
    日本伊势原东海大学医学部内科，消化内科和肝病科。
3
    日本伊势原东海大学医学院血液肿瘤科。

抽象

乙型肝炎病毒（HBV）产生大量完整和不完整的病毒颗粒。除了作为感染性粒子的病毒颗粒外，这些颗粒的功能仍然难以捉摸。已经揭示细胞外囊泡（EV）具有生物学功能。从HBV感染患者收集直径小于100nm的EV。这些囊泡包含完整和不完整的病毒粒子和外泌体，最近已被证明是细胞间通讯的关键。在此，研究了外来体，完整颗粒和不完全颗粒对靶细胞的影响。这些颗粒被单核细胞/巨噬细胞内吞，主要起到上调PD-L1的作用。 EV的功能和组成受核苷酸逆转录酶抑制剂（NRTIs）的影响，表明EV参与HBV肝炎的发病机制和NRTI治疗的患者的临床过程。

结论：
    30596665
DOI：
    10.1371 / journal.pone.0205886

StephenW

https://journals.plos.org/ploson ... ournal.pone.0205886