血浆lncRNA小核仁RNA宿主基因1在肝细胞癌诊断中的应用。

StephenW

Mol Med Rep. 2018 Jul 30. doi: 10.3892/mmr.2018.9336. [Epub ahead of print]
Diagnostic utility of plasma lncRNA small nucleolar RNA host gene 1 in patients with hepatocellular carcinoma.
Gao S1, Xu X2, Wang Y3, Zhang W1, Wang X2.
Author information

1
    Department of Oncology, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar, Heilongjiang 161021, P.R. China.
2
    Department of Clinical Laboratory, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar, Heilongjiang 161021, P.R. China.
3
    Medical School of Nanchang University, Nanchang, Jiangxi 330031, P.R. China.

Abstract

Long non‑coding RNAs (lncRNAs) offer great potential as cancer biomarkers. Owing to the limited sensitivity and specificity of α‑fetoprotein (AFP) for the diagnosis of hepatocellular carcinoma (HCC), the present study used an lncRNA microarray to screen aberrantly expressed lncRNAs in HCC tissues. Subsequently, the expression profile of the target lncRNAs was investigated in plasma from patients with HCC or hepatitis B virus‑positive chronic hepatitis and cirrhosis (HCH), as well as from healthy volunteers. A total of six aberrantly expressed lncRNAs were identified in HCC tissues and corresponding normal tissues, from which only small nucleolar RNA host gene 1 (SNHG1) expression in HCC tissues demonstrated a good correlation with those in plasma from HCC patients. Subsequent analysis revealed that high plasma SNHG1 expression levels were correlated with tumor size, TNM stage and AFP levels. Receiver operating characteristic analysis demonstrated that SNHG1 yields an excellent diagnostic ability to differentiate between patients with HCC and unaffected control patients, which was superior to that of AFP. The combination of SNHG1 with AFP may be able to distinguish HCC from HCH or healthy volunteers with the area under the curve values of 0.86 and 0.97, respectively. In summary, it was demonstrated that plasma SNHG1 has great potential as a sensitive and reliable biomarker for the diagnosis of HCC.

PMID:
    30066898
DOI:
    10.3892/mmr.2018.9336

StephenW

Mol Med Rep.2018 7月30日doi：10.3892 / mmr.2018.9336。 [提前打印]
血浆lncRNA小核仁RNA宿主基因1在肝细胞癌诊断中的应用。
高S1，徐X2，王Y3，张文1，王X2。
作者信息

1
    齐齐哈尔医科大学附属第三医院肿瘤科，齐齐哈尔，黑龙江161021，中国。
2
    齐齐哈尔医学院第三附属医院检验科，黑龙江齐齐哈尔161021，中国。
3
    南昌大学医学院，江西南昌330031，中国。

抽象

长的非编码RNA（lncRNA）作为癌症生物标志物提供了巨大的潜力。由于甲胎蛋白（AFP）对肝细胞癌（HCC）诊断的敏感性和特异性有限，本研究使用lncRNA微阵列筛选HCC组织中异常表达的lncRNA。随后，在来自患有HCC或乙型肝炎病毒阳性慢性肝炎和肝硬化（HCH）的患者以及来自健康志愿者的血浆中研究靶标lncRNA的表达谱。在HCC组织和相应的正常组织中共鉴定出6种异常表达的lncRNA，其中在HCC组织中仅有小的核仁RNA宿主基因1（SNHG1）表达与来自HCC患者的血浆中的那些表现出良好的相关性。随后的分析显示高血浆SNHG1表达水平与肿瘤大小，TNM分期和AFP水平相关。受试者工作特征分析表明，SNHG1对HCC患者和未受影响的对照患者具有良好的诊断能力，优于AFP。 SNHG1与AFP的组合可能能够区分HCC与HCH或健康志愿者，其曲线下面积分别为0.86和0.97。总之，已证明血浆SNHG1作为诊断HCC的敏感且可靠的生物标志物具有巨大潜力。

结论：
    30066898
DOI：
    10.3892 / mmr.2018.9336