急性乙型肝炎感染免疫反应动力学

StephenW

Dynamics of the Immune Response in Acute Hepatitis B Infection
Femke Stelma Sophie B Willemse Robin Erken Annikki de Niet Marjan J Sinnige Karel van Dort Hans L Zaaijer Ester M M van Leeuwen Neeltje A Kootstra Hendrik W Reesink
Open Forum Infectious Diseases, Volume 4, Issue 4, 1 October 2017, ofx231, https://doi.org/10.1093/ofid/ofx231
Published:
20 December 2017


Abstract
Background

Acute hepatitis B virus infection in adults is generally self-limiting but may lead to chronicity in a minority of patients.
Methods

We included 9 patients with acute hepatitis B virus (HBV) infection and collected longitudinal follow-up samples. Natural killer (NK) cell characteristics were analyzed by flowcytometry. HBV-specific T-cell function was analyzed by in vitro stimulation with HBV peptide pools and intracellular cytokine staining.
Results

Median baseline HBV DNA load was 5.12 log IU/mL, and median ALT was 2652 U/mL. Of 9 patients, 8 cleared HBsAg within 6 months whereas 1 patient became chronically infected. Early time points after infection showed increased CD56bright NK cells and an increased proportion of cells expressing activation markers. Most of these had normalized at week 24, while the proportion of TRAIL-positive CD56bright NK cells remained high in the chronically infected patient. In patients who cleared HBV, functional HBV-specific CD8+ and CD4+ responses could be observed, whereas in the patient who developed chronic infection, only low HBV-specific T-cell responses were observed.
Conclusions

NK cells are activated early in the course of acute HBV infection. Broad and multispecific T-cell responses are observed in patients who clear acute HBV infection, but not in a patient who became chronically infected.

StephenW

急性乙型肝炎感染免疫反应动力学
Femke Stelma Sophie B Willemse Robin Erken Annikki de Niet Marjan J Sinnige Karel van Dort Hans L Zaaijer Ester M M van Leeuwen Neeltje A Kootstra Hendrik W Reinsink
开放论坛传染病，卷4，第4期，2017年10月1日，ofx231，https://doi.org/10.1093/ofid/ofx231
发布时间：
2017年12月20日


抽象
背景

成人急性乙型肝炎病毒感染通常是自限性的，但可能导致少数病人的慢性病。
方法

我们包括9例急性乙型肝炎病毒（HBV）感染者，并收集纵向随访样本。通过流式细胞术分析自然杀伤（NK）细胞特征。通过用HBV肽库和细胞内细胞因子染色的体外刺激来分析HBV特异性T细胞功能。
结果

HBV DNA基线中位数为5.12 log IU / mL，ALT中位数为2652 U / mL。 9例患者中，6个月内8例HBsAg清除，1例患者慢性感染。感染后的早期时间点显示CD56bright NK细胞增加，表达活化标记的细胞比例增加。其中大多数在24周时已经正常化，而慢性感染患者中TRAIL阳性CD56bright NK细胞的比例仍然很高。在清除HBV的患者中，可观察到功能性HBV特异性CD8 +和CD4 +应答，而在发生慢性感染的患者中，仅观察到低的HBV特异性T细胞应答。
结论

NK细胞在急性HBV感染过程中被激活。在清除急性HBV感染的患者中观察到广泛的和多特异性的T细胞应答，但是在患有慢性感染的患者中不存在。

StephenW

https://academic.oup.com/ofid/article/4/4/ofx231/4767777

antiHBVren

运动，能激活抗癌急先锋NK细胞(图)
http://hbvhbv.info/forum/thread-1696536-1-1.html

运动，能让身体恢复！

StephenW

在急性乙型肝炎高峰期，ALT可达3970 iu / l