Dynamics of the Immune Response in Acute Hepatitis B Infection
Femke Stelma Sophie B Willemse Robin Erken Annikki de Niet Marjan J Sinnige Karel van Dort Hans L Zaaijer Ester M M van Leeuwen Neeltje A Kootstra Hendrik W Reesink
Open Forum Infectious Diseases, Volume 4, Issue 4, 1 October 2017, ofx231, https://doi.org/10.1093/ofid/ofx231
Published:
20 December 2017
Abstract
Background
Acute hepatitis B virus infection in adults is generally self-limiting but may lead to chronicity in a minority of patients.
Methods
We included 9 patients with acute hepatitis B virus (HBV) infection and collected longitudinal follow-up samples. Natural killer (NK) cell characteristics were analyzed by flowcytometry. HBV-specific T-cell function was analyzed by in vitro stimulation with HBV peptide pools and intracellular cytokine staining.
Results
Median baseline HBV DNA load was 5.12 log IU/mL, and median ALT was 2652 U/mL. Of 9 patients, 8 cleared HBsAg within 6 months whereas 1 patient became chronically infected. Early time points after infection showed increased CD56bright NK cells and an increased proportion of cells expressing activation markers. Most of these had normalized at week 24, while the proportion of TRAIL-positive CD56bright NK cells remained high in the chronically infected patient. In patients who cleared HBV, functional HBV-specific CD8+ and CD4+ responses could be observed, whereas in the patient who developed chronic infection, only low HBV-specific T-cell responses were observed.
Conclusions
NK cells are activated early in the course of acute HBV infection. Broad and multispecific T-cell responses are observed in patients who clear acute HBV infection, but not in a patient who became chronically infected. 作者: StephenW 时间: 2018-1-11 16:22
急性乙型肝炎感染免疫反应动力学
Femke Stelma Sophie B Willemse Robin Erken Annikki de Niet Marjan J Sinnige Karel van Dort Hans L Zaaijer Ester M M van Leeuwen Neeltje A Kootstra Hendrik W Reinsink
开放论坛传染病，卷4，第4期，2017年10月1日，ofx231，https://doi.org/10.1093/ofid/ofx231
发布时间：
2017年12月20日