慢性乙型肝炎病毒感染者自发清除乙型肝炎病毒过程中CD8 +记

StephenW

Eur Rev Med Pharmacol Sci. 2017 Oct;21(20):4675-4679.
Study on the distribution of CD8+ memory T cell subsets and IFN-γ level during the spontaneous clearance of hepatitis B virus in patients with chronic hepatitis B virus infection.Ma XJ1, Chen XF, Chen WL, Chen R, Huang J, Luo XD, Liao JY, Chen XP.
Author information
1Department of Infectious Diseases, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangdong, China. [email protected].

AbstractOBJECTIVE: To study the alteration of CD8+ memory T cell subsets under different immune statuses during the spontaneous clearance of hepatitis B virus (HBV) in Chinese patients with chronic HBV infection.
PATIENTS AND METHODS: We analyzed Chinese patients with chronic HBV infection including 10 patients with Hepatitis B surface Antigen (HBsAg) spontaneous seroconversion, 25 patients with Hepatitis B virus e Antigen (HBeAg) spontaneous seroconversion, 25 patients with chronic hepatitis B (CHB), and 25 chronic HBV carriers. The CD8+ T cells in peripheral blood were isolated, and flow cytometry was used to determine the percent change of CD8+ T memory cell subsets. ELISA was used to measure the levels of Interferon-γ (IFN-γ) secretion from CD8+ T cells.
RESULTS: (1) The percentage of CD8+ TN cells in peripheral blood was lower in the HBsAg seroconversion group than in the HBeAg seroconversion group (p<0.01), and higher in the CHB group and chronic HBV carrier group (p<0.01, 0.01); (2) The percentage of CD8+ TEM-2 memory T cells in peripheral blood was higher in the HBsAg seroconversion group than the HBeAg seroconversion group (p<0.05), CHB group, and chronic HBV carrier group (p<0.01, 0.01); (3) The percentage of CD8+ TEM-1 and CD8+ TCM cells in peripheral blood was higher in the CHB group and HBV carrier group than the HBsAg seroconversion group and HBeAg group, but there were no significant differences between groups (p>0.05); (4) IFN-γ production from CD8+ T cells in peripheral blood was higher in the HBsAg seroconversion group than the HBeAg seroconversion group (p<0.05), CHB group, and chronic HBV carrier group (p<0.05, 0.01).
CONCLUSIONS: The consistent increase of CD8+ TEM-2 cell subsets may be an important cause of spontaneous clearance of HBV. The disorder of CD8+ memory T cell differentiation may be an important mechanism of chronic HBV infection.


PMID:29131246

StephenW

Eur Rev Med Pharmacol Sci。 2017年10月; 21（20）：4675-4679。
慢性乙型肝炎病毒感染者自发清除乙型肝炎病毒过程中CD8 +记忆T细胞亚群和IFN-γ水平的分布研究
马×1 1，陈晓峰1，陈伟1，陈蕊1，黄，1，罗晓东2，廖建英1，
作者信息

1
    广东省医学科学院广东总医院感染科，广东省[email protected]。

抽象
目的：

研究中国慢性HBV感染者自发清除乙型肝炎病毒（HBV）期间不同免疫状态下CD8 +记忆T细胞亚群的变化。
患者和方法：

我们分析了中国慢性乙型肝炎患者，包括10例乙型肝炎表面抗原（HBsAg）自发性血清转换，25例乙型肝炎病毒e抗原（HBeAg）自发血清转换，25例慢性乙型肝炎（CHB）和25例慢性乙型肝炎载体。分离外周血CD8 + T细胞，流式细胞仪检测CD8 + T记忆细胞亚群变化。使用ELISA来测量CD8 + T细胞的干扰素-γ（IFN-γ）分泌水平。
结果：

（1）HBsAg血清转换组外周血CD8 + TN细胞百分比低于HBeAg血清转换组（p <0.01），CHB组和慢性HBV携带组高于对照组（P <0.01,0.01）。 （2）HBsAg血清转换组外周血CD8 + TEM-2记忆T细胞百分比高于HBeAg血清转换组（p <0.05），CHB组和慢性HBV载体组（p <0.01,0.01）。 （3）CHB组和HBV载体组外周血CD8 + TEM-1和CD8 +细胞比例高于HBsAg血清学转换组和HBeAg组，但组间比较差异无统计学意义（P> 0.05）。 （4）HBsAg血清转换组外周血CD8 + T细胞产生的IFN-γ水平高于HBeAg血清转换组（p <0.05），CHB组和慢性HBV携带者组（p <0.05,0.01）。
结论：

CD8 + TEM-2细胞亚群的持续增加可能是HBV自发清除的重要原因。 CD8 +记忆T细胞分化障碍可能是慢性HBV感染的重要机制。

结论：
    29131246

antiHBVren

中华临床医师杂志 电子版 2014 年8 月第8 卷第16 期 Chin J Clinicians Electronic Edition ,August 15,2014,Vol.8,No.16 ·临床论著·
慢性乙型肝炎患者CD8 记忆T 细胞的 检测分析
熊清芳 黄平 赵磊 赵红 王华利 冯晓宁 杨永峰 +
【摘要】 目的 探讨慢性乙型肝炎患者和急性乙型肝炎恢复期患者CD8 记忆T细胞的产生及 分布特性，分析其临床意义。方法 流式细胞术检测16例急性乙型肝炎恢复期患者、26例慢性乙型 肝炎患者和18名健康人的外周血CD8+记忆T细胞亚群，以初始T细胞、中心记忆T细胞 （TCM ）、效 + 应记忆T细胞 （TEM ）、终末分化效应细胞（TTEM ）占总计数CD8 细胞数的百分比表示。结果 与健 康组、急性乙型肝炎恢复期组相比，慢性乙型肝炎组患者CD8+初始T细胞明显增加 （P ＜0.001 ）； TCM 、TEM 细胞亚群分布频率显著降低 （P ＜0.001 ）。急性乙型肝炎恢复期组与健康组相比，TCM升 高（t ＝2.34 ，P ＝0.03 ）。高病毒载量组慢性乙型肝炎TTEM百分比比中低病毒载量高（t ＝2.43，P ＝0.02 ）。 无论肝炎活动期还是静止期，初始T细胞、TCM 、TEM 、TTEM 百分比差异均无统计学意义。结论 慢 性乙型肝炎患者和急性乙型肝炎恢复期患者CD8+记忆T淋巴细胞亚群的比例存在明显差异，可能与 体内抗原长期存在以及刺激的程度相关。从而导致清除病毒的能力下降，慢性化的形成。 【关键词】 肝炎，乙型，慢性； 免疫； CD8阳性T淋巴细胞； 记忆T细胞
Reciprocal changes of naive and effector/memory CD8+ T lymphocytes in chronic hepatitis B virus * infection Xiong Qingfang , Huang Ping, Zhao Lei, Zhao Hong, Wang Huali, Feng Xiaoning, Yang * Yongfeng. Department of Liver Disease, The Second Hospital of Nanjing, Affiliated to Medical School of South-East University, Nanjing 210003, China Corresponding author: Yang Yongfeng, Email: tongxqf@1 63.com
【Abstract 】 Objective To determine the production and frequencies of CD8+ memory T cells from patients with chronic infection of hepatitis B virus HBV and subjects recovering from acute hepatitis B. Methods A total of 26 patients with chronic hepatitis B who were hospitalized in the second Hospital of Nanjing from November 2011 to April 2014 and 16 subjects recovering from acute hepatitis B during same time period were recruited in the study. The CD8+ memory T cell subsets were analyzed by CD8, CD27, CCR7 and CD45RA staining and flow cytometry. Results In comparison to healthy subjects and patients recovering from acute hepatitis B, chronic hepatitis B patients showed higher percentages of naive CD8+ T lymphocytes, and lower percentages of Central memory T cell

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