Eur Rev Med Pharmacol Sci. 2017 Oct;21(20):4675-4679.
Study on the distribution of CD8+ memory T cell subsets and IFN-γ level during the spontaneous clearance of hepatitis B virus in patients with chronic hepatitis B virus infection.Ma XJ1, Chen XF, Chen WL, Chen R, Huang J, Luo XD, Liao JY, Chen XP. Author information 1Department of Infectious Diseases, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangdong, China. [email protected].
AbstractOBJECTIVE: To study the alteration of CD8+ memory T cell subsets under different immune statuses during the spontaneous clearance of hepatitis B virus (HBV) in Chinese patients with chronic HBV infection.
PATIENTS AND METHODS: We analyzed Chinese patients with chronic HBV infection including 10 patients with Hepatitis B surface Antigen (HBsAg) spontaneous seroconversion, 25 patients with Hepatitis B virus e Antigen (HBeAg) spontaneous seroconversion, 25 patients with chronic hepatitis B (CHB), and 25 chronic HBV carriers. The CD8+ T cells in peripheral blood were isolated, and flow cytometry was used to determine the percent change of CD8+ T memory cell subsets. ELISA was used to measure the levels of Interferon-γ (IFN-γ) secretion from CD8+ T cells.
RESULTS: (1) The percentage of CD8+ TN cells in peripheral blood was lower in the HBsAg seroconversion group than in the HBeAg seroconversion group (p<0.01), and higher in the CHB group and chronic HBV carrier group (p<0.01, 0.01); (2) The percentage of CD8+ TEM-2 memory T cells in peripheral blood was higher in the HBsAg seroconversion group than the HBeAg seroconversion group (p<0.05), CHB group, and chronic HBV carrier group (p<0.01, 0.01); (3) The percentage of CD8+ TEM-1 and CD8+ TCM cells in peripheral blood was higher in the CHB group and HBV carrier group than the HBsAg seroconversion group and HBeAg group, but there were no significant differences between groups (p>0.05); (4) IFN-γ production from CD8+ T cells in peripheral blood was higher in the HBsAg seroconversion group than the HBeAg seroconversion group (p<0.05), CHB group, and chronic HBV carrier group (p<0.05, 0.01).
CONCLUSIONS: The consistent increase of CD8+ TEM-2 cell subsets may be an important cause of spontaneous clearance of HBV. The disorder of CD8+ memory T cell differentiation may be an important mechanism of chronic HBV infection.
中华临床医师杂志 电子版 2014 年8 月第8 卷第16 期 Chin J Clinicians Electronic Edition ,August 15,2014,Vol.8,No.16 ·临床论著·
慢性乙型肝炎患者CD8 记忆T 细胞的 检测分析
熊清芳 黄平 赵磊 赵红 王华利 冯晓宁 杨永峰 +
【摘要】 目的 探讨慢性乙型肝炎患者和急性乙型肝炎恢复期患者CD8 记忆T细胞的产生及 分布特性，分析其临床意义。方法 流式细胞术检测16例急性乙型肝炎恢复期患者、26例慢性乙型 肝炎患者和18名健康人的外周血CD8+记忆T细胞亚群，以初始T细胞、中心记忆T细胞 （TCM ）、效 + 应记忆T细胞 （TEM ）、终末分化效应细胞（TTEM ）占总计数CD8 细胞数的百分比表示。结果 与健 康组、急性乙型肝炎恢复期组相比，慢性乙型肝炎组患者CD8+初始T细胞明显增加 （P ＜0.001 ）； TCM 、TEM 细胞亚群分布频率显著降低 （P ＜0.001 ）。急性乙型肝炎恢复期组与健康组相比，TCM升 高（t ＝2.34 ，P ＝0.03 ）。高病毒载量组慢性乙型肝炎TTEM百分比比中低病毒载量高（t ＝2.43，P ＝0.02 ）。 无论肝炎活动期还是静止期，初始T细胞、TCM 、TEM 、TTEM 百分比差异均无统计学意义。结论 慢 性乙型肝炎患者和急性乙型肝炎恢复期患者CD8+记忆T淋巴细胞亚群的比例存在明显差异，可能与 体内抗原长期存在以及刺激的程度相关。从而导致清除病毒的能力下降，慢性化的形成。 【关键词】 肝炎，乙型，慢性； 免疫； CD8阳性T淋巴细胞； 记忆T细胞
Reciprocal changes of naive and effector/memory CD8+ T lymphocytes in chronic hepatitis B virus * infection Xiong Qingfang , Huang Ping, Zhao Lei, Zhao Hong, Wang Huali, Feng Xiaoning, Yang * Yongfeng. Department of Liver Disease, The Second Hospital of Nanjing, Affiliated to Medical School of South-East University, Nanjing 210003, China Corresponding author: Yang Yongfeng, Email: tongxqf@1 63.com
【Abstract 】 Objective To determine the production and frequencies of CD8+ memory T cells from patients with chronic infection of hepatitis B virus HBV and subjects recovering from acute hepatitis B. Methods A total of 26 patients with chronic hepatitis B who were hospitalized in the second Hospital of Nanjing from November 2011 to April 2014 and 16 subjects recovering from acute hepatitis B during same time period were recruited in the study. The CD8+ memory T cell subsets were analyzed by CD8, CD27, CCR7 and CD45RA staining and flow cytometry. Results In comparison to healthy subjects and patients recovering from acute hepatitis B, chronic hepatitis B patients showed higher percentages of naive CD8+ T lymphocytes, and lower percentages of Central memory T cell