健康正常体重的女性60-天卧床休息对NAFLD肝指的影响

StephenW

Effect of enforced physical inactivity induced by 60-d of bed rest on hepatic markers of NAFLD in healthy normal-weight women

    Floriane Rudwill1,2,†,
    Audrey Bergouignan3,†,
    Caroline Gastebois4,
    Guillemette Gauquelin-Koch5,
    Etienne Lefai4,
    Stéphane Blanc1,2 and
    Chantal Simon4,*

DOI: 10.1111/liv.12743

This article is protected by copyright. All rights reserved.

Issue
Cover image for Vol. 34 Issue 10
Liver International


Author Information

    1    University of Strasbourg, IPHC, Strasbourg, France
    2    CNRS, UMR 7178, Strasbourg, France
    3    Anschutz Health and Wellness Center, University of Colorado Anschutz Medical Campus, Aurora, CO, USA
    4    CARMEN, INSERM U1060, University of Lyon 1, INRA1235, CRNH-RA, Oullins, France
    5    CNES, Paris, France

    †    F. Rudwill and A. Bergouignan contributed equally to this work as first authors.

* Corresponding author and person to whom reprint request should be addressed:
Chantal Simon, MD
Service d'Endocrinologie, Diabète, Nutrition
Centre Hospitalier Lyon Sud
165 Chemin du Grand Revoyet
69600 Oullins, France
Phone: +33426235917 - Fax: +33426235916
e-mail: [email protected]

    This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi: 10.1111/liv.12743


Abstract
Background

Physical inactivity leads to a cluster of metabolic disorders that have been associated with non-alcoholic fatty liver diseases.
Aim

To test whether physical inactivity increases hepatic biomarkers of non-alcoholic fatty liver diseases.
Methods

Sixteen normal-weight healthy women (body mass index=21.2±0.5 kg.m−2) were studied under controlled energy balance conditions during a previous 60-day bed-rest with (n=8) or without (n=8) a combined aerobic/resistive exercise protocol. We retrospectively used stored samples to measure plasma hepatic markers, i.e. steatosis-related alanine and aspartate transaminases, cytokeratin 18 and angiopoietin-like 3, at baseline, after 30 and 60 days of bed-rest. Fasting insulin and triglycerides were measured at baseline and after 30 days of bed-rest. Two indexes were calculated, one combining alanine, aspartate transferase and cytokeratin 18 and another cytokeratin 18, homeostasis model assessement–insulin resistance and aspartate transferase.
Results

Sixty days of bed-rest increased all hepatic markers (p<0.05 for all) and the two indexes (p<0.01 for both). Exercise significantly reduced the elevation in aspartate transaminase, cytokeratin 18 and both indexes (p<0.02 for all) but not the increase in alanine transferase and angiopoietin-like 3. Changes between baseline and 30 days of bed rest in triglycerides were positively associated with changes in aspartate transferase (R2=0.28, p=0.04) suggesting a role of hypertriglyceridemia in the alteration of liver metabolism under inactive conditions.
Conclusions

Physical inactivity increases, independent of fat mass, hepatic markers of steatosis and steatohepatitis. Regular exercise can limit these physical inactivity-induced metabolic alterations. Future studies need to elucidate the underlying mechanisms.

This article is protected by copyright. All rights reserved.

StephenW

致60-D卧床休息对NAFLD的健康正常体重的女性肝指标执行体力活动的影响

    Floriane Rudwill1,2，†，
    奥黛丽Bergouignan3，†，
    卡罗琳Gastebois4，
    Guillemette高奎林-Koch5，
    艾蒂安Lefai4，
    斯特凡Blanc1,2和
    尚塔尔Simon4，*

DOI：10.1111/ liv.12743

这篇文章是受版权保护的。版权所有。

问题
封面图片的卷。 34问题10
肝国际


作者信息

    斯特拉斯堡，IPHC，法国斯特拉斯堡大学1
    2 CNRS，UMR7178，斯特拉斯堡，法国
    3安舒茨卫生与健康中心，科罗拉多安舒茨医学校区大学，极光，CO，USA
    4 CARMEN，INSERM U1060，里昂1，INRA1235，CRNH-RA大学，Oullins，法国
    5 CNES，法国巴黎

    †F. Rudwill和A. Bergouignan同等贡献这项工作作为第一作者。

*通讯作者，并亲自为之转载请求应该得到解决：
尚塔尔西门，MD
服务D'Endocrinologie，糖尿病反义，营养
中心医院南基里昂
165舍曼杜大Revoyet
69600 Oullins，法国
电话：33426235917 - 传真：33426235916
电子邮件：[email protected]

    这篇文章已被接受发表，并接受全面的同行评审，但经过审稿，排版，分页和校对过程中，这可能会导致这个版本和记录的版本之间的差异一直没有。请引用这篇文章的DOI：10.1111/ liv.12743


抽象
背景

身体活动导致代谢紊乱的簇已与非酒精性脂肪肝疾病。
目的

为了测试身体活动是否增加的非酒精性脂肪肝疾病的肝的生物标志物。
方法：

十六个体重正常的健康妇女（身体质量指数=21.2±0.5千克。米-2）在控制能量平衡的条件在之前60天的卧床休息与进行了研究（N =8）或无（N =8）结合有氧/抗阻力运动协议。我们回顾用于存储样品测量血浆肝标志物，即脂肪变性相关丙氨酸，天门冬氨酸转氨酶，细胞角蛋白18和血管生成素样3，在基线，经过卧床休息30到60天。空腹胰岛素和甘油三酯测定在基线和后卧床休息30天。两项指标分别计算，1相结合的丙氨酸，天门冬氨酸转移酶和细胞角蛋白18和另一个细胞角蛋白18，稳态模型assessement胰岛素抵抗和天门冬氨酸转移。
结果

床上休息六十天增加肝脏的所有标记（P均<0.05）和两项指标（P均<0.01）。运动显著降低了抬高谷草转氨酶，细胞角蛋白18和两个指数（P <0.02为所有），但不增加丙氨酸转移酶和血管生成素样和基线之间3.变化卧床休息甘油三酯30天内正相关变化有关在天冬氨酸转移酶（R2 =0.28，P = 0.04），表明高甘油三酯在肝脏代谢不活跃的条件下的改变的作用。
结论

体力活动增加，独立的脂肪量，脂肪肝和脂肪性肝炎的肝标记。有规律的运动可以限制这些体力活动引起的代谢改变。未来的研究需要阐明的基本机制。

这篇文章是受版权保护的。版权所有。