B型肝炎病毒的影响，对人类精液参数和精子DNA 完整性

StephenW

Zhonghua Nan Ke Xue. <http://www.ncbi.nlm.nih.gov/pubmed/24218942&gt; 2013
Oct;19(10):896-8.

[Effects of hepatitis B virus on human semen parameters and sperm DNA
integrity].

[Article in Chinese]

Liu H
<http://cirrus.mail-list.com/hepatitis-b/75794230.html&gt;, Geng CH
<http://cirrus.mail-list.com/hepatitis-b/32735492.html&gt;, Wang W
<http://cirrus.mail-list.com/hepatitis-b/34788436.html&gt;, Xiao KL
<http://cirrus.mail-list.com/hepatitis-b/96076075.html&gt;, Xiong LK
<http://cirrus.mail-list.com/hepatitis-b/75397770.html&gt;, Huang YX
<http://cirrus.mail-list.com/hepatitis-b/65645011.html&gt;, Yang XL
<http://cirrus.mail-list.com/hepatitis-b/14028991.html&gt;, Li J
<http://cirrus.mail-list.com/hepatitis-b/32755643.html&gt;.

Source: http://www.ncbi.nlm.nih.gov/pubmed/24218942

Department of Reproductive Healthcare, Shenzhen Bao'an Maternity and
Child Healthcare Hospital, Shenzhen, Guangdong 518133, China.
[email protected]

Abstract

OBJECTIVE:

To investigate the effects of hepatitis B virus (HBV) in semen on human
semen parameters and sperm DNA integrity.

METHODS:

We detected HBV DNA in the semen samples of 153 HBsAg-seropositive
patients by real-time fluorescence quantitative PCR and calculated the
sperm nuclear DNA fragmentation index (DFI) by sperm chromatin dispersion
(SCD) assay. We compared the semen parameters between the HBV DNA-
positive group (A, n = 43) and HBV DNA-negative group (B, n = 110) and
analyzed the correlation of sperm DFI with the number of HBV DNA copies
in the semen.

RESULTS:

HBV DNA was detected in 43 (28.1%) of the 153 semen samples. No
statistically significant differences were observed in age, semen volume
and sperm concentration between groups A and B (P >0.05). Compared with
group B, group A showed significantly decreased sperm viability ([58.0
+/- 18.8]% vs [51.4 +/-17.1]%, P<0.05), progressively motile sperm ([29.6
+/- 13.3]% vs [24.5 +/- 10.1]%, P<0.05), average straight-line velocity
([23.7 +/- 4.0] microm/s vs [19.9 +/- 4.5 ] microm/s, P<0.01) and average
path velocity ([26.5 +/- 7.0] microm/s vs [23.4 +/- 5.3] microm/s,
P<0.01), but remarkably decreased sperm DFI ([19.3 +/- 8.0]% vs [24.2 +/-
9.4]%, P<0.01). The number of HBV DNA copies in semen exhibited a
significant positive correlation with sperm DFI (r = 0.819, P < 0.01).

CONCLUSION:

HBV DNA in semen is not significantly associated with the number of
sperm, but may affect sperm viability, velocity and DFI. There is a load-
effect relationship between the number of HBV DNA copies in semen and
sperm nuclear DNA integrity.

StephenW

中华男科学杂志。 <http://www.ncbi.nlm.nih.gov/pubmed/24218942&gt; 2013
年10月，19 （10） :896 - 8 。

[ B型肝炎病毒的影响，对人类精液参数和精子DNA

完整性] 。

[中国]

刘洪
<http://cirrus.mail-list.com/hepatitis-b/75794230.html&gt; ，耿CH
<http://cirrus.mail-list.com/hepatitis-b/32735492.html&gt; ，王文
<http://cirrus.mail-list.com/hepatitis-b/34788436.html&gt; ，肖KL
<http://cirrus.mail-list.com/hepatitis-b/96076075.html&gt; ，熊LK
<http://cirrus.mail-list.com/hepatitis-b/75397770.html&gt; ，黄裕
<http://cirrus.mail-list.com/hepatitis-b/65645011.html&gt; ，杨XL
<http://cirrus.mail-list.com/hepatitis-b/14028991.html&gt; ，李江
<http://cirrus.mail-list.com/hepatitis-b/32755643.html&gt; 。

资料来源： http://www.ncbi.nlm.nih.gov/pubmed/24218942

深圳市宝安区妇产和生殖保健部，
妇幼保健院，广东深圳， 518133 ，中国。
[email protected]

抽象

目的：

精液对人体B型肝炎病毒（HBV）的影响进行调查
精液参数和精子DNA完整性。

方法：

我们发现153例HBsAg血清学阳性的精液样本中HBV DNA
患者的实时荧光定量PCR和计算
精子核DNA片段化指数（ DFI ） ，通过精子染色质分散
（ SCD ）检测。我们比较了精液参数之间的HBV DNA
阳性对照组（ A组，n = 43 ）和HBV DNA阴性组（ B组，n = 110 ）和
，精子DFI与HBV DNA拷贝数的相关性进行了分析
在精液中。

结果：

HBV-DNA检测153精液样本中有43 （28.1％） 。没有
统计学显着性差异，观察在年龄，精液量
和精子浓度组之间的A和B （P> 0.05 ） 。相对于
B组， A组显着下降，精子活力（ 58.0
+ / -  18.8 ％与51.4 + / -17.1 ％ ，P <0.05） ，逐步游动精子（ 29.6
+ /  - 13.3 ％比24.5 + / -  10.1 ％ ，P <0.05） ，平均直线速度
（ 23.7 + /  - 4.0 ] MICROM / s比19.9 + /  -  4.5 ] MICROM / S ， P <0.01 ），平均
路径速度（ 26.5 + /  - 7.0 ] MICROM /秒和23.4 + / -  5.3 ] MICROM /秒，
P <0.01 ） ，但显着降低精子DFI （ 19.3 + /  - 8.0 ％与24.2 + /  -
9.4 ％ ，P <0.01 ） 。精液中HBV DNA拷贝数呈现
显着的正相关关系，与精子DFI相关（r = 0.819， P <0.01） 。

结论：

精液中HBV DNA的数量没有显着关联
精子，但可能会影响精子的活力，速度和DFI 。有载荷
精液中HBV DNA的数量之间的因果关系复制，
精子核DNA完整性。