Department of Reproductive Healthcare, Shenzhen Bao'an Maternity and
Child Healthcare Hospital, Shenzhen, Guangdong 518133, China. [email protected]
Abstract
OBJECTIVE:
To investigate the effects of hepatitis B virus (HBV) in semen on human
semen parameters and sperm DNA integrity.
METHODS:
We detected HBV DNA in the semen samples of 153 HBsAg-seropositive
patients by real-time fluorescence quantitative PCR and calculated the
sperm nuclear DNA fragmentation index (DFI) by sperm chromatin dispersion
(SCD) assay. We compared the semen parameters between the HBV DNA-
positive group (A, n = 43) and HBV DNA-negative group (B, n = 110) and
analyzed the correlation of sperm DFI with the number of HBV DNA copies
in the semen.
RESULTS:
HBV DNA was detected in 43 (28.1%) of the 153 semen samples. No
statistically significant differences were observed in age, semen volume
and sperm concentration between groups A and B (P >0.05). Compared with
group B, group A showed significantly decreased sperm viability ([58.0
+/- 18.8]% vs [51.4 +/-17.1]%, P<0.05), progressively motile sperm ([29.6
+/- 13.3]% vs [24.5 +/- 10.1]%, P<0.05), average straight-line velocity
([23.7 +/- 4.0] microm/s vs [19.9 +/- 4.5 ] microm/s, P<0.01) and average
path velocity ([26.5 +/- 7.0] microm/s vs [23.4 +/- 5.3] microm/s,
P<0.01), but remarkably decreased sperm DFI ([19.3 +/- 8.0]% vs [24.2 +/-
9.4]%, P<0.01). The number of HBV DNA copies in semen exhibited a
significant positive correlation with sperm DFI (r = 0.819, P < 0.01).
CONCLUSION:
HBV DNA in semen is not significantly associated with the number of
sperm, but may affect sperm viability, velocity and DFI. There is a load-
effect relationship between the number of HBV DNA copies in semen and
sperm nuclear DNA integrity. 作者: StephenW 时间: 2013-11-22 14:29