The natural YMDD mutations of hepatitis B virus in Western China.

StephenW

我不明白为什么有自然YMMD突变?

Scand J Infect Dis. 2011 Aug 19. [Epub ahead of print]

The natural YMDD mutations of hepatitis B virus in Western China.

Zhao J, Guo Y, Yan Z, Liang P, Zhang J, Liu Y.

Source

State Key Laboratory of Cancer Biology, School of Pharmacy, the Fourth
Military Medical University , Xi'an , China.

Abstract

Abstract Background: To provide a scientific basis for clinical genetic
diagnosis before lamivudine treatment in hepatitis B virus (HBV) patients,
the rate of natural YMDD mutations in Western China and the correlations
between YMDD mutations and several factors were investigated in this study.
Methods: A pyrosequencing approach for detecting YMDD mutations was first
developed. The sensitivity of the pyrosequencing approach was determined by
assaying polymerase chain reaction (PCR) products generated from 10-fold
serial dilutions of HBV DNA and PCR products from mixed control plasmids
with different ratios. Natural YMDD mutations in Western China were
evaluated by analyzing the clinical samples from HBV patients who had no
experience of using lamivudine, and the existence of YMDD mutants was
further confirmed by real-time PCR. Results: With the developed
pyrosequencing approach, YMDD mutations could be determined within 2 h
after PCR amplification. About 10 copies of HBV DNA per reaction were
required to obtain sufficient PCR products to produce clear and accurate
pyrosequencing patterns. The pyrosequencing approach developed had the
capacity to detect minor mutants; most HBV mutants in samples were minor
ones. The rate of natural YMDD mutations in Western China was 15.56%. The
hepatitis B e antigen (HBeAg) level in serum was correlated with YMDD
mutations. Conclusions: In Western China, natural YMDD mutations occur at a
rate of 15.56%, and it is suggested that pyrosequencing be performed to
detect YMDD mutations before lamivudine treatment in patients with a
chronic HBV infection.

StephenW

谷歌翻译
不是100％准确，仅供参考使用。

Scand传染病杂志2011年08月19日。 [检索提前打印]

在中国西部的自然乙肝病毒YMDD变异。

严中伟，赵俊，郭Ÿ，梁P，张军，刘禹

来源

肿瘤生物学国家重点实验室，药学院，第四
军医大学，西安，中国。

摘要

摘要背景：为了提供科学依据为临床遗传
乙型肝炎病毒（HBV）患者在拉米夫定治疗前诊断，
中国西部自然YMDD变异率和相关性
在这项研究中YMDD变异之间的几个因素进行了调查。
方法：一个用于检测YMDD变异的焦磷酸测序方法首次
发展。焦磷酸测序方法的灵敏度是由
化验聚合酶链反应（PCR）技术产生的产品，从10倍
从混合控制质粒HBV DNA和PCR产物的连续稀释
不同的比率。在中国西部的天然YMDD变异
评估分析谁没有乙肝患者的临床标本
使用拉米夫定的经验和存在YMDD突变
实时PCR进一步证实。结果：与发达
焦磷酸测序方法可确定在2 h内，YMDD变异
PCR扩增后。约10份HBV - DNA，每个反应
需要获得足够的PCR产物，产生清晰和准确的
焦磷酸测序的模式。焦磷酸测序的方法开发了
检测轻微的突变体的能力;大多数乙肝病毒样本中的突变体小
的。在中国西部的自然YMDD变异率分别为15.56％。 “
乙型肝炎e抗原（HBeAg）血清中的水平与YMDD变异相关
突变。结论：在中国西部天然YMDD变异发生在
率15.56％，这是建议，焦磷酸测序进行
前检测患者用拉米夫定治疗YMDD变异
慢性乙肝病毒感染。