标题: The natural YMDD mutations of hepatitis B virus in Western China. [打印本页] 作者: StephenW 时间: 2011-8-22 22:19 标题: The natural YMDD mutations of hepatitis B virus in Western China.
我不明白为什么有自然YMMD突变?
Scand J Infect Dis. 2011 Aug 19. [Epub ahead of print]
The natural YMDD mutations of hepatitis B virus in Western China.
Zhao J, Guo Y, Yan Z, Liang P, Zhang J, Liu Y.
Source
State Key Laboratory of Cancer Biology, School of Pharmacy, the Fourth
Military Medical University , Xi'an , China.
Abstract
Abstract Background: To provide a scientific basis for clinical genetic
diagnosis before lamivudine treatment in hepatitis B virus (HBV) patients,
the rate of natural YMDD mutations in Western China and the correlations
between YMDD mutations and several factors were investigated in this study.
Methods: A pyrosequencing approach for detecting YMDD mutations was first
developed. The sensitivity of the pyrosequencing approach was determined by
assaying polymerase chain reaction (PCR) products generated from 10-fold
serial dilutions of HBV DNA and PCR products from mixed control plasmids
with different ratios. Natural YMDD mutations in Western China were
evaluated by analyzing the clinical samples from HBV patients who had no
experience of using lamivudine, and the existence of YMDD mutants was
further confirmed by real-time PCR. Results: With the developed
pyrosequencing approach, YMDD mutations could be determined within 2 h
after PCR amplification. About 10 copies of HBV DNA per reaction were
required to obtain sufficient PCR products to produce clear and accurate
pyrosequencing patterns. The pyrosequencing approach developed had the
capacity to detect minor mutants; most HBV mutants in samples were minor
ones. The rate of natural YMDD mutations in Western China was 15.56%. The
hepatitis B e antigen (HBeAg) level in serum was correlated with YMDD
mutations. Conclusions: In Western China, natural YMDD mutations occur at a
rate of 15.56%, and it is suggested that pyrosequencing be performed to
detect YMDD mutations before lamivudine treatment in patients with a
chronic HBV infection. 作者: StephenW 时间: 2011-8-22 22:21