Mutations in HBV virus DNA from patients with coexisting HBsAg and anti-

StephenW

<http://www.journalofclinicalvirology.com/article/PIIS1386653211002861/abstract?rss=yes>

Journal of Clinical Virology

Article in Press

Mutations in hepatitis B virus DNA from patients with coexisting HBsAg and
anti-HBs

Yu Chen, Fuchu Qian, Quan Yuan, Xuefen Li, Wei Wu, Xichao Guo, Lanjuan Li

Received 25 February 2011; received in revised form 6 May 2011; accepted 8
July 2011. published online 16 August 2011. Corrected Proof

Abstract

Background

The serological markers with coexistence of hepatitis B surface antigen
(HBsAg) and antibody to HBsAg (anti-HBs) of hepatitis B virus (HBV)
infection were rare pattern. The virological significance, immune response
and clinical outcome of these patients remain largely unknown.

Objectives

This research explores the relationship between this serological profile
and HBV genome variants.

Study design

We studied 35 patients both carrying HBsAg and anti-HBs (group I), and 70
patients with HBsAg positive but anti-HBs negative (group II, served as
control). The HBV genome sequences were obtained by direct sequencing of
polymerase chain reaction (PCR) products.

Results

The amino acid (aa) variation within major hydrophilic region (MHR),
especially in the first loop (aa124-137) of “a” determinant in group I
is significantly higher than those in group II. The aa variation of
cytotoxic lymphocyte (CTL) epitope in HBsAg (aa87–aa95) in group I is
also significantly higher than that in group II. Interestingly, the basal
core promoter (BCP) double mutations (A1762T/G1764A) in group I is
significantly higher than those in group II as well.

Conclusions

In patients with HBV infection, the coexistence of HBsAg and anti-HBs is
associated with an increased aa variability in several key areas of HBV
genome. The molecular characteristic of HBV in HBsAg and anti-HBs positive
patients is distinct and worth further studies.



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StephenW

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临床病毒学杂志

文章在印刷中

乙肝病毒DNA突变, 在HBsAg 和 抗- HBs 共存的患者

陈渝，府中钱，袁泉，李雪芬，吴炜，郭西潮，李兰娟

接获252011年2月，在经修订的2011年5月6日收到接受8
2011年7月。在网上公布2011年8月16日。更正的证明

摘要

背景

血清学标志物, 与乙肝表面抗原（HBsAg）和
表面抗体（抗- HBs）并存的感染罕见的格局。病毒学意义，免疫反应这些患者的临床结果仍是未知。

目标

本研究文件探讨此血清HBV基因变异体之间的关系。

研究设计

我们研究了35例患者都携带乙肝表面抗原和抗- HBs（I组）和70
患者HBsAg阳性，但抗- HBs阴性（Ⅱ组，担任
控制）。 HBV基因组序列直接测序，获得
聚合酶链反应（PCR）产品。

结果

氨基酸（AA）主要亲水区（MHR）的变化范围内，
特别是在第一循环的“A”组的行列式（aa124- 137）我
明显比第二组的高。机管局的变化
细胞毒性淋巴细胞（CTL）的抗原表位组在HBsAg（aa87- aa95）我是
也明显高于第二组。有趣的是，基
核心启动子（BCP）双突变组（A1762T/G1764A）我是
显着高于在第二组，以及。

结论

在HBV感染的患者，HBsAg和抗- HBs共存
与增加的AA在几个关键领域的HBV变异
基因组。 HBsAg和抗- HBs阳性HBV的分子特征
患者是不同的，值得进一步研究。