标题: Mutations in HBV virus DNA from patients with coexisting HBsAg and anti- [打印本页] 作者: StephenW 时间: 2011-8-19 22:03 标题: Mutations in HBV virus DNA from patients with coexisting HBsAg and anti-
Mutations in hepatitis B virus DNA from patients with coexisting HBsAg and
anti-HBs
Yu Chen, Fuchu Qian, Quan Yuan, Xuefen Li, Wei Wu, Xichao Guo, Lanjuan Li
Received 25 February 2011; received in revised form 6 May 2011; accepted 8
July 2011. published online 16 August 2011. Corrected Proof
Abstract
Background
The serological markers with coexistence of hepatitis B surface antigen
(HBsAg) and antibody to HBsAg (anti-HBs) of hepatitis B virus (HBV)
infection were rare pattern. The virological significance, immune response
and clinical outcome of these patients remain largely unknown.
Objectives
This research explores the relationship between this serological profile
and HBV genome variants.
Study design
We studied 35 patients both carrying HBsAg and anti-HBs (group I), and 70
patients with HBsAg positive but anti-HBs negative (group II, served as
control). The HBV genome sequences were obtained by direct sequencing of
polymerase chain reaction (PCR) products.
Results
The amino acid (aa) variation within major hydrophilic region (MHR),
especially in the first loop (aa124-137) of “a” determinant in group I
is significantly higher than those in group II. The aa variation of
cytotoxic lymphocyte (CTL) epitope in HBsAg (aa87–aa95) in group I is
also significantly higher than that in group II. Interestingly, the basal
core promoter (BCP) double mutations (A1762T/G1764A) in group I is
significantly higher than those in group II as well.
Conclusions
In patients with HBV infection, the coexistence of HBsAg and anti-HBs is
associated with an increased aa variability in several key areas of HBV
genome. The molecular characteristic of HBV in HBsAg and anti-HBs positive
patients is distinct and worth further studies.