[Research]Circulating microRNAs in hepatitis B virus–infected patients

StephenW

<http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2893.2011.01443.x/abstract>

Circulating microRNAs in hepatitis B virus–infected patients

F. Ji1, B. Yang1, X. Peng2, H. Ding3, H. You4, P. Tien1

Article first published online: 7 MAR 2011
DOI: 10.1111/j.1365-2893.2011.01443.x
© 2011 Blackwell Publishing Ltd
Issue

Journal of Viral Hepatitis
Early View (Articles online in advance of print)

Summary.  MicroRNAs (miRNAs) are stably present in human serum. The
relationship between circulating miRNAs and hepatitis B virus (HBV) infected
liver disease has not been previously reported. Applied Biosystems array-based
miRNA expression profiling was performed on pooled sera obtained from identified
groups of chronic asymptomatic carriers (ASC), patients with chronic hepatitis B
(CHB) and HBV-associated acute-on-chronic liver failure (ACLF), as well as
healthy controls (HC). Nine miRNAs were verified in more clinical samples by
RT-PCR. The correlation between miRNAs expression and the relationship between
miRNA levels and clinical characteristics was analysed. Results showed that
circulating miRNAs were detected in all disease and control samples, and their
numbers increased with symptom severity, from 37 in HC, 77 in ASC, 101 in CHB,
to 135 in ACLF. The expression levels of most miRNAs were also up-regulated in
HBV-infected patients when compared to HC. Expression of the liver-specific
miR-122 was significantly up-regulated in HBV-infected patients. Concomitant
regulation of miRNAs not in clusters was disrupted by HBV infection. However,
such disruption was not observed for miRNAs in paralogous clusters. Furthermore,
the level of miRNAs in the CHB serum was up-regulated most in hepatitis B e
antigen–positive patients. The expression levels of miR-122 and miR-194
correlated negatively with the age of patients with CHB or ACLF. Functional
analysis showed that miR-122 could inhibit HBV replication in Huh7 and HepG2
cells. In all, our study revealed that a number of miRNAs were differentially
expressed during HBV infection and underscored the potential importance of
miR-122 in the infection process.



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StephenW

乙型肝炎病毒感染的患者循环的microRNA

1楼祭，二杨一，十，彭二，丁三阁下，阁下您4，第1天

文章首先发表时间：2011年3月7日
分类号：10.1111/j.1365-2893.2011.01443.x
© 2011布莱克韦尔出版有限公司
发行

作者：病毒性肝炎
厄尔利维尤（第预先在网上打印）

综述。小分子RNA（miRNAs）是人血清中稳定存在。该
微RNA之间的循环和乙型肝炎病毒（HBV）感染的关系
肝病没有被报告过。应用生物系统公司的基于阵列
miRNA的表达谱进行汇集从血清得到确认
慢性无症状携带者（ASC），患者组，慢性乙型肝炎
（CHB）和乙肝病毒相关的急性上慢性肝功能衰竭（ACLF），以及
（HC）的健康对照组。九，验证了微RNA在更多的临床样本的
RT - PCR检测。 miRNA的表达之间的关系和相关性
miRNA的水平和临床特点进行了分析。结果表明：
循环miRNA的检测和控制所有疾病中的样品，其
人数增加与症状严重程度，从37的HC，77升序，慢性乙型肝炎101，
以135 ACLF。大部分miRNA的表达水平也上调中
乙肝病毒感染的患者相比，慧聪。表达的肝脏特异性
的miR - 122显着上调，在乙肝病毒感染者管理。伴随
miRNA的调控不被打乱了集群HBV感染。然而，
这种干扰是无法观察到的旁系集群的miRNA。此外，
对慢性乙型肝炎患者血清中miRNA的水平上调乙型肝炎大多数电子邮件
抗原阳性患者。中miR - 122和miR - 194的表达水平
呈负相关的慢性乙肝患者的年龄或ACLF。功能
分析显示了miR - 122能够抑制乙型肝炎病毒复制和在Huh7肝癌
细胞。总之，我们的研究显示，有一些差异的miRNA
乙肝病毒感染过程中表示，并强调潜在的重要性
的miR - 122在感染的过程。