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http://7thspace.com/headlines/374712/hepatitis_b_virus_x_protein_impedes_the_dna_repair_via_itsassociation_with_transcription_factor_tfiih.html
Hepatitis B virus (HBV) infections play an important role in the development of hepatocellular carcinoma (HCC). HBV X protein (HBx) is a multifunctional protein that can modulate various cellular processes and plays a crucial role in the pathogenesis of HCC.
HBx is known to interact with DNA helicase components of TFIIH, a basal transcriptional factor and an integral component of DNA excision repair.
Results: In this study, the functional relevance of this association was further investigated in the context to DNA repair. By site-directed mutagenesis HBx's critical residues for interaction with TFIIH were identified.
Similarly, TFIIH mutants lacking ATPase domain and the conserved carboxyl-terminal domain failed to interact with HBx. Yeast and mammalian cells expressing HBxwt conferred hypersensitivity to UV irradiation, which is interpreted as a basic deficiency in nucleotide excision repair.
HBxmut120 (Glu to Val) was defective in binding to TFIIH and failed to respond to UV.
Conclusions: We conclude that HBx may act as the promoting factor by inhibiting DNA repair causing DNA damage and accumulation of errors, thereby contributing to HCC development.
Author: Ishtiaq QadriKaneez FatimaHany Hafiz
Credits/Source: BMC Microbiology 2011, 11:48 |
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