标题: Hepatitis B virus X protein impedes the DNA repair via itsassociation with trans [打印本页] 作者: StephenW 时间: 2011-3-5 08:14 标题: Hepatitis B virus X protein impedes the DNA repair via itsassociation with trans
HBx is known to interact with DNA helicase components of TFIIH, a basal transcriptional factor and an integral component of DNA excision repair.
Results: In this study, the functional relevance of this association was further investigated in the context to DNA repair. By site-directed mutagenesis HBx's critical residues for interaction with TFIIH were identified.
Similarly, TFIIH mutants lacking ATPase domain and the conserved carboxyl-terminal domain failed to interact with HBx. Yeast and mammalian cells expressing HBxwt conferred hypersensitivity to UV irradiation, which is interpreted as a basic deficiency in nucleotide excision repair.
HBxmut120 (Glu to Val) was defective in binding to TFIIH and failed to respond to UV.
Conclusions: We conclude that HBx may act as the promoting factor by inhibiting DNA repair causing DNA damage and accumulation of errors, thereby contributing to HCC development.