以下是引用hemming在2004-6-2 17:40:00的发言：
--------------------------------------------------------------------------------
来源：中国医药网 时间：2004.03.30
新华社信息北京３月３０日电 美国宾夕法尼亚州立医学院的研究人员开发出了一种微型载体，能够找到并杀死小鼠肝脏中８０％的乙肝病毒。
该医学院教授加里·克劳森和他
的研究小组开发出了ＳＮＩＰＡＡ盒式载体，其中包含一种双倍剂量的特殊核酶，称为交互作用锤头核酶。核酶是核糖核酸（ＲＮＡ）的片断，像酶一样可导致ＲＮＡ其他片断的化学变化和分裂。ＲＮＡ对“生命之书”ＤＮＡ的复制极为关键，同时也是病毒复制的关键。
ＳＮＩＰＡＡ盒以脂质体形式包装（脂质体是在身体内释放药物的重要工具），然后脂质体又反过来为蛋白质所改造，这样它就能找出乙肝病毒在其中进行复制的肝脏细胞。
找到肝脏细胞后，ＳＮＩＰＡＡ盒就被释放入细胞并直达细胞核，在那里产生活性核酶。核酶通过切断病毒ＲＮＡ破坏其产生蛋白质的能力，从而使其不能发挥作用。蛋白质是病毒复制的关键。
克劳森等人首先确定乙肝病毒ＲＮＡ中的最佳目标区域，然后用培养的细胞检验ＳＮＩＰＡＡ盒的有效性。在细胞培养组织中，他们发现，在３-５天的时间里，含有双倍剂量核酶的ＳＮＩＰＡＡ盒比含单倍剂量的ＳＮＩＰＡＡ盒能杀死更多的病毒。
克劳森说：“这是为数不多的在动物活体实验中减少乙肝病毒产生的有效疗法之一。虽然这次针对的是乙肝病毒，但我们有针对性地包装核酶的方法也应适用于其他病毒疗法。”（完）
BR80559263.0 附上英文：http://www.news-medical.net/?id=93
News-Medical.Net
Tiny Ribozyme package could be future treatment of Hepatitis B virusPosted By: News-Medical in Pharmaceutical NewsPublished: Monday, 22-Mar-2004 Printer Friendly
Email to a Friend
Penn State College of Medicine researchers have developed a tiny package that searches for and destroys up to 80 percent of hepatitis B virus in the livers of mice.
“This marks one of the few successful in vivo, or in-animal, models of an effective therapy to reduce the production of hepatitis B virus,” said Gary Clawson, M.D., Ph.D., professor of pathology, biochemistry and molecular biology, Penn State College of Medicine. “Although this work focused on hepatitis B virus, our method of targeting and packaging ribozymes should also be applicable to the development of therapies to fight other viruses.”
The study was published March 5 in the online version of the journal, Molecular Therapy, the official journal of the American Society of Gene Therapy, and will appear in the journal’s April print edition.
Hepatitis B virus (HBV) attacks the liver and can cause lifelong infection, liver cancer and, eventually, death. Although HBV is treated with drugs, it cannot be cured. The chronic disease affects about 1.25 million Americans, 20 percent to 30 percent of whom acquired the virus in childhood. HBV is transmitted via blood or sexual activity, but also may be transmitted from mother to child during childbirth. Once infected, the virus continues to reproduce in the liver.
Clawson, who is also director of the Jake Gittlen Cancer Research Center at Penn State Milton S. Hershey Medical Center, and his team developed the SNIPAA cassette, which contains a double-dose of a special type of ribozyme called a trans-acting hammerhead ribozyme. Ribozymes are ribonucleic acid (RNA) segments that, like enzymes, cause chemical changes or splitting in other RNA segments. RNA, which is critical to the replication of DNA - life’s instruction book - also is critical to the replication of viruses. The SNIPAA cassette was packaged in liposomes, typical vehicles for delivering drugs in the body, and the liposomes in turn were modified with proteins so that they would seek out the liver cells where the HBVs replicate.
Once at the liver cell, the SNIPAA cassette package is released into the cell and finds its way to the cell nucleus, where the active ribozymes are produced. The ribozymes destroy the viral RNA’s ability to produce proteins by cleaving, or cutting, the viral RNAs, rendering them useless. Proteins are critical to virus replication.
First, Clawson and co-workers used proprietary in vitro selection techniques to identify the best target sites in HBV RNA, and then used cells in culture to test the effectiveness of the cassette. In the cell cultures, he found that the SNIPAA cassette containing a double-dose of ribozymes eliminated more of the virus over a period of three to five days than did cassettes containing a single dose of the ribozymes.
Specialized transgenic mice, which contain the HBV in their DNA and which are chronic carriers of HBV, were treated with the tiny HBV-fighting packages. Clawson and co-workers chose the dosage and schedule of the drug delivery to the mice based on their cell culture work. Studies with the transgenic mice were performed with John Morrey, Ph.D., at Utah State, under the auspices of an NIH contract which supports testing of antiviral reagents.
“The treatment effects were quite dramatic,” Clawson said. “We recorded a greater than 80 percent reduction in the HBV liver DNA, meaning far less virus was being produced, and staining for the virus using antibodies also showed a dramatic decrease in residual viral production. This is significant because there are so few examples of successful in vivo applications of ribozymes against bona fide naturally-occurring, disease-causing organisms.”
Clawson believes this cassette is more effective than previous ones for a number of reasons. First, the best target sites were chosen using a “library selection” process. Second, the ribozyme cassette is engineered to cut itself into pieces, thereby freeing the HBV-targeted ribozymes from extraneous sequences that could interfere with their activity. In addition, Clawson’s cassette included two distinct trans-acting ribozymes, whereas previous versions included only one. With two trans-acting hammerhead ribozymes in the SNIPAA cassette, twice the amount of “medicine” was present and was delivered over a longer period.
Co-authors on the study were: Wei-Hua Pan, Pin Xin, Departments of Pathology and Biochemistry and Molecular Biology, The Gittlen Cancer Research Institute, Penn State Milton S. Hershey Medical Center, and John D. Morrey, Institute for Antiviral Research, Utah State University.
This work was supported by a research and development contract with Biosan Laboratories/Hexal AG and by a contract from the National Institutes of Health. | 
楼主: 天狼星
发表于 2004-12-3 23:26
