analysis of immune-related genes in dendritic cells from hepatitis B surface ant

StephenW

My opinion only: this research supports the use of immunogenic complex in a therapeutic vaccine being developed.只是我个人的看法：这个研究支持治免疫原性复杂在一个开发中疗性疫苗中的使用。

J Med Virol. 2011 Jan;83(1):78-87.

Transcriptional analysis of immune-related genes in dendritic cells from
hepatitis B surface antigen (HBsAg)-positive transgenic mice and
regulation of Fc gamma receptor IIB by HBsAg-anti-HBs complex.

Yao X, Wang XY, Zhao C, Sun SH, Meng ZF, Zhang JM, Xu JQ, Xie YH, Yuan ZH,
Wen YM.

Key Laboratory of Medical Molecular Virology, Ministry of
Education/Ministry of Health, Shanghai Medical College, Fudan University,
Shanghai, China.

Abstract
To study the gene expression profiles in dendritic cells (DCs) from a
hepatitis B surface antigen (HBsAg) positive host, transcriptional
analysis of bone marrow -derived DCs from a lineage of HBsAg transgenic
mice (#59) was compared to DCs from normal mice. Among the immune-related
genes, 12 were up-regulated, and 14 were down-regulated in transgenic mice
relative to those of normal mice. The up-regulated genes include genes
encoding immunoglobulin, histocompatibility 2 (K region), and several
complement component genes, while the down-regulated genes include the
TAP1 (transporters associated with antigen processing gene-1), interferon
induced gene (Ifi203), chemokine (C-X-C) ligands and
leukocyte-immunoglobulin-like genes, Lck-interacting transmembrane adaptor
genes and histocompatibility 2 (Q region and T region). Since an
immunogenic complex containing HBsAg-anti-HBs has been used as a
therapeutic vaccine for clinical trial in chronic hepatitis B patients,
DCs from #59 were incubated with immunogenic complex compared to those
incubated with HBsAg alone. The immune-related six genes up-regulated with
immunogenic complex treatment were Fcgr2b, Cxcl2, Fth1, Clec4n, Lilrb4,
and Dbh, with Fcgr2b (Fc gamma receptor IIB) being the highest
up-regulated gene. Interestingly, levels of Fcgr2b were found up-regulated
in patients with chronic hepatitis B undergoing immunogenic complex
immunization, which returned to baseline when immunization was
discontinued. In conclusion, by transcriptional analysis, immunogenic
complex induced up-regulation of Fcgr2b expression both in dendritic cells
from an HBsAg transgenic mouse model and peripheral B cells from patients
with chronic hepatitis B, which indicates that Fcgr2b is one of the key
molecules up-regulated by immunogenic complex.

J. Med. Virol. 83:78-87, 2011. © 2010 Wiley-Liss, Inc.
PMID: 21108342 [PubMed - in process]
                           

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医志病毒学杂志。 2011年一月，83（1）:78 - 87。

转录分析树突状细胞的免疫相关基因
乙型肝炎表面抗原（HBsAg）阳性和转基因小鼠
通过调节伽马Fc的乙肝表面抗原，抗- HBs复合物受体防爆。

姚x 王xy，赵C，孙sh，孟zf，张jm，徐jq，谢yh，袁zh，
温ym。

重点实验室，医学分子病毒学教育部
教育/卫生部，上海医学院，复旦大学，
上海，中国。

摘要
研究从一个在树突状细胞的基因表达图谱（区议会）
乙型肝炎表面抗原（HBsAg）阳性的主机，转录
骨髓来源的分析，从区议会的HBsAg转基因谱系
鼠（＃59）进行了比较，从正常小鼠的DC。其中免疫相关
基因，12个表达上调，14位向下调节的转基因小鼠
相对于正常小鼠的。在上调基因包括基因
编码免疫球蛋白，组织相容性2（九龙区），和几个
补体基因，而下调的基因包括：
TAP1（抗原处理基因- 1相关的运输），干扰素
诱导基因（Ifi203），趋化因子（趋）配体和
白细胞免疫球蛋白样基因，Lck相互作用跨膜适配器
组织相容性基因和2（问区和T区）。由于一
乙肝表面抗原免疫复合物，抗- HBs已被用作一
治疗性疫苗对慢性乙型肝炎患者的临床试验，
从＃59区议会分别与相比，那些复杂的免疫原性
培养与HBsAg孤单。免疫有关的六个基因上调与
免疫综合治疗的Fcgr2b，Cxcl2，Fth1，Clec4n，Lilrb4，
和胸径，与Fcgr2b（财委会γ受体IIB级）为最高等级
上调基因。有趣的是，Fcgr2b水平被认为上调
在慢性乙型肝炎患者接受免疫原性复杂
免疫接种，这回到基线时，免疫接种
终止。总之，用转录分析，免疫原性
复杂的诱导上调表达的Fcgr2b无论是在树突状细胞
从表面抗原的转基因小鼠模型，从患者外周血B细胞
慢性乙型肝炎，这表明Fcgr2b是其中的关键
分子组成，受复杂的免疫原性。

j的医科大学。病毒学杂志。 83:78-87，2011。 © 2010威利-利斯公司
结论：21108342 [PubMed的 - 过程]
                        

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StephenW

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wonder4078

翻译一下嘛