|
- 现金
- 62111 元
- 精华
- 26
- 帖子
- 30437
- 注册时间
- 2009-10-5
- 最后登录
- 2022-12-28
|
<http://7thspace.com/headlines/33 ... ing_retrovirus.html>
Efficient inhibition of hepatitis B virus replication by hepatitis delta virus
ribozymes delivered by targeting retrovirus
Hepatitis delta virus (HDV) ribozyme is an attractive molecular tool that can
specifically recognize and catalyze the self-cleavage of the viral RNA
phosphodiester backbone. However, a major obstacle in the medical application
of the HDV ribozyme is the lack of specificity in the delivery of the ribozyme
to defined target cells.
Results: The objective of this study was to determine whether retroviral
vectors can deliver the HDV ribozyme into the target cells and to elucidate
whether HDV ribozyme plays a role in hepatitis B virus (HBV) replication.
In our study, the transduction of helper-free pseudotyped retrovirus, which
showed a broad host range, in human hepatoma cells was performed under 2
conditions, that is, in the presence of polymerized human serum albumin (pHSA)
and in the absence of pHSA. The transduction ability in the presence of pHSA
was higher than in the absence of pHSA.
Moreover, HBsAg and HBeAg levels after transductions with pHSA were
significantly lower than those in the absence of pHSA, thus indicating that
the recombinant retrovirus had HBV-specific cleavage activity and targeted
HepG2215 cells.
Conclusions: These data suggest that this system provides a new approach for
targeting hepatocytes and has a great potential in gene therapy for HBV
infection.
Author: Chuan-Xi WangYan-Qin LuPeng QiLong-Hua ChenJin-Xiang Han
Credits/Source: Virology Journal 2010, 7:61 |
-
总评分: 现金 + 10
查看全部评分
|
发表于 2010-3-19 20:54
