Suppression of p38 mitogen-activated protein kinase inhibits hepatitis B virus

StephenW

<http://www3.interscience.wiley.com/journal/120174773/abstract>

Journal of Viral Hepatitis
Early View (Articles online in advance of print)
Published Online: 22 Jan 2008
© 2010 Blackwell Publishing Ltd

Suppression of p38 mitogen-activated protein kinase inhibits hepatitis B virus
replication in human hepatoma cell: the antiviral role of nitric oxide

W.-W. Chang 1 , I.-J. Su 2 , M.-D. Lai 3 , W.-T. Chang 3 , W. Huang 4 and
H.-Y. Lei 1,5
  1 Department of Basic Medical Sciences, College of Medicine, National Cheng
Kung University, Tainan ;  2 Division of Clinical Research, National Health
Research Institutes, Tainan ;  3 Department of Biochemistry and Molecular
Biology, College of Medicine, National Cheng Kung University, Tainan ;  4
Department of Medical Laboratory Science and Biotechnology, College of
Medicine, National Cheng Kung University, Tainan ; and  5 Department of
Microbiology & Immunology, College of Medicine, National Cheng Kung
University, Tainan, Taiwan

Correspondence to Huan-Yao Lei PhD, Department of Microbiology & Immunology,
College of Medicine, National Cheng Kung University, Tainan, 70101, Taiwan.
E-mail: [email protected]

Copyright 2008 The Authors Journal compilation 2008 Blackwell Publishing Ltd


ABSTRACT
Summary. The role of the p38 mitogen-activated protein kinase (MAPK) pathway
in hepatitis B virus (HBV) replication was investigated in this study. After
transient transfection with HBV plasmid, p38 MAPK, but not JNK or ERK1/2, was
significantly phosphorylated in human hepatoma cell Huh7. Interestingly, HBV
proteins and RNA synthesis were significantly inhibited by a specific
inhibitor of p38 MAPK, SB203580, in a dose-dependent manner. Intracellular
core-associated DNA, extracellular virion-associated DNA and covalently closed
circular DNA were also significantly inhibited by SB203580. Further results
showed the antiviral role of nitric oxide (NO) on the suppression of HBV
replication and downregulation of p38 MAPK phosphorylation. In conclusion,
these results suggested that suppression of phosphorylation of p38 MAPK by
inhibitor or NO could inhibit intracellular HBV replication.

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Received July 2007; accepted for publication September 2007
DIGITAL OBJECT IDENTIFIER (DOI)
10.1111/j.1365-2893.2007.00968

jsmscym

看不懂，请翻译一下。

小民百姓

NO可以抑制肝细胞内病毒复制？一氧化氮还有这功效？

tonychant

剩饭再炒也不好吃

StephenW

回复 小民百姓 的帖子

这个研究报告是旧(2008)，很少进一步研究NO和HBV.