Hepatitis B viral X protein

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Hepatitis B viral X protein alters the biological features and expressions of
DNA repair enzymes in LO2 cells

Authors: Cheng, Bin1; Zheng, Yaochu1; Guo, Xiaorong1; Wang, Ying1; Liu,
Chunyan1
Source: Liver International, Volume 30, Number 2, February 2010 , pp.
319-326(8)
Publisher: Blackwell Publishing

This study aimed at examining the effects of hepatitis B viral X protein (HBx)
on the biological features and the expression of DNA repair enzymes in
non-tumour human hepatic LO2 cells in vitro. Methods:
The HBx gene was transfected into LO2 cells to establish stably HBx-expressing
LO2/HBx cells. The morphological features, cell growth, cell cycle, apoptosis
and colony formation of LO2/HBx cells, vector-transfected LO2/pcDNA3.1 cells
and unmanipulated LO2 cells were studied. The expressions of DNA repair
enzymes and DNA oxidative stress-related 8-hydroxydeoxyguanosine (8-OHdG) were
determined by a real-time quantitative polymerase chain reaction assay and
high-performance liquid chromatography coupled with electrochemical detection
respectively. Results:
In comparison with controls, significant morphological changes, faster growth,
higher frequency of cells at the S phase, but lower at G0/G1 and M/G2 phases,
a lower frequency of natural cell apoptosis and a higher percentage of colony
formation were observed in the LO2/HBx cells. Furthermore, significantly
higher levels of intracellular 8-OHdG and lower levels of human DNA
glycosylase α (hMYHα) mRNA transcripts, but no significant change in human
8-oxoguanine DNA glycosylase 1 (hOGG1), were detected in the LO2/HBx cells.
Conclusions:
Our data indicated that HBx promoted growth and malignant transformation of
non-tumour hepatic LO2 cells in vitro, which was associated with the
downregulation of hMYHα expression and accumulation of mutagenic DNA adduct
8-OHdG.

Document Type: Research article
DOI: 10.1111/j.1478-3231.2009.02167.x
Affiliations: 1: Department of Gastroenterology and Hepatology, Tongji
Hospital, Tongji Medical College, Huazhong University of Science and
Technology, Wuhan, China