Whole-Blood Counting Immunoassay ...

liver411

Journal of Clinical Microbiology, September 2004, p. 4250-4252, Vol. 42, No.
9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4250-4252.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Whole-Blood Counting Immunoassay as a Short-Turnaround Test for Detection of
Hepatitis B Surface Antigen, Anti-Hepatitis C Virus Antibodies, and
Anti-Treponema pallidum Antibodies

Toyoichiro Kudo,1* Aiko Kido,1 Yukiko Nishiyama,1 Hiroshi Koganeya,1 Takako
Okuda,1 Motoshige Nabeshima,2 Yoshitsugu Iinuma,1 and Satoshi Ichiyama1
Department of Laboratory Medicine,1 Department of Gastroenterology and
Hepatology, Graduate School of Medicine, Kyoto University, Kyoto, Japan2

Received 21 October 2003/ Returned for modification 7 January 2004/ Accepted
6 May 2004

Whole-blood samples were used for a counting immunoassay (CIA) with the aim
of developing a short- turnaround test. After optimization of the CIA,
hepatitis B surface antigen (HBsAg), anti-hepatitis C virus antibodies
(anti-HCV), and anti-Treponema pallidum antibodies (anti-TP) were detected
as efficiently as by an enzyme immunoassay (EIA) with serum samples. The
correlations between whole-blood CIA and serum EIA were 99.8, 97.1, and
99.4% for HBsAg, anti-HCV, and anti-TP, respectively. Whole-blood CIA may be
of value when rapid screening of many samples is required.

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* Corresponding author. Mailing address: Clinical Laboratory, Division of
Infectious Diseases, Kyoto University Hospital, 54 Kawahara Shogoin,
Sakyoku, Kyoto 606-8507, Japan. Phone: 81-75-751-4914. Fax: 81-75-751-3233.
E-mail: [email protected].

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Journal of Clinical Microbiology, September 2004, p. 4250-4252, Vol. 42, No.
9
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.9.4250-4252.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved