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发表于 2003-10-7 00:31
Chinese Journal of Digestive Diseases
Published on behalf of the Chinese Society of Gastroenterology and the
Chinese Medical Association Shanghai Branch
Print ISSN: 1443-9611
Online ISSN: 1443-9573
Frequency: Quarterly
Current Volume: 4
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Volume 4: Issue 3
Hepatoprotective effect of a curcumin/absinthium compound in experimental
severe liver injury
F MAROTTA
YR SHIELD
T BAMBA
Y NAITO
E MINELLI
M YOSHIOKA
Abstract
OBJECTIVE:
A preliminary in vitro study with hepatocyte culture showed that
concentrations as low as 10 µg/mL of PN-M001 are able to significantly
mitigate CCl4 hepatocyte damage (P < 0.05) comparable to 100 µg/mL
silymarin, and 100 µg/mL proved to be more protective than either silymarin
100 µg/mL or glycyrrhizin 10 µg/mL (P < 0.05).
METHODS:
Wistar rats were allocated into three groups: (A) 0.1 mL/100 g body weight
(BW) mixture of CCl4 in olive oil (1 : 1 v/v) subcutaneous injection twice
daily for 4 weeks; (B) as A, plus oral administration of 50 mg/kg of K-17.22
dissolved in 5% glucose; (C) as B but with PN-M001 given 1 week after the
first injection of CCl4. Rats were killed at the end of the study and blood
and liver samples were obtained.
RESULTS:
When compared with a control, group A showed a significant decrease of
glutathione (GSH;>45%, P < 0.001) and oxidized GSH (GSSG; P < 0.01) liver
content, a lower liver wet weight (P < 0.01) together with an increase of
both transaminases (>15-fold, P < 0.001) whereas groups B and C both showed
only a mild increase in transaminases (<4-fold, P < 0.05). Group A showed a
significant decrease of Y-protein fraction and of GST activity, as tested by
both substrates (P < 0.01 vs control). However, both these parameters were
reverted to normal by PN-M001 (P < 0.05 vs A).
CONCLUSIONS:
These preliminary data suggest that PN-M001 exerts a highly protective and
prolonged effect (either preventive or therapeutic) on GSH depletion in
CCl4-induced liver injury, which suggests its potential use in the clinical
setting.
Article Type: Original Article
Page range: 122 - 127
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