基於乙型肝炎病毒相關特異性基因的肝細胞癌預後特徵構建

StephenW

基於乙型肝炎病毒相關特異性基因的肝細胞癌預後特徵構建與驗證
王磊#1 2、邱曼曼#3、吳麗麗 4、李澤興 5、Xinyi Meng 6、Lu He 7、Bing Yang 8
隸屬關係
隸屬關係

     1個
     天津市第二人民醫院，天津 300192
     2個
     天津市肝病研究所，天津 300192
     3個
     南開大學生命科學學院, 天津, 300071
     4個
     武警後勤學院, 天津 300000
     5個
     天津大學生命科學學院, 天津, 300072
     6個
     天津醫科大學基礎醫學院細胞生物學教研室，天津 300070
     7
     天津醫科大學基礎醫學院解剖與組織學教研室，天津 300070
     8個
     天津醫科大學基礎醫學院細胞生物學教研室，天津 300070 [email protected].

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貢獻相等。

     PMID：36474267 DOI：10.1186/s13027-022-00470-y

抽象的

背景：肝細胞癌（HCC）是一種常見的原發性肝癌，是癌症相關死亡的主要原因之一。 乙型肝炎病毒 (HBV) 感染是 HCC 的重要危險因素。 因此，本研究旨在探討HBV陽性HCC相關特異性基因在HCC中的預後作用。

方法：從癌症基因組圖譜（TCGA）、國際癌症基因組聯盟（ICGC）和基因表達綜合數據庫（GEO）三個數據庫下載HCC相關數據。 進行單變量 Cox 回歸分析和 LASSO Cox 回歸分析以建立風險評分。 多變量Cox回歸分析和生存分析確定了獨立的預後指標。

結果：通過對差異表達基因（DEGs）的交叉分析，我們確定了106個重疊的DEGs，它們很可能是HBV陽性HCC相關特異性基因。 這 106 個 DEGs 在 213 個 GO 術語和 8 個 KEGG 通路中顯著豐富。 其中，選擇了11個最佳基因構建Risk評分，Risk評分是HCC的獨立預後因素。 高危 HCC 患者的 OS 較差。 此外，五種免疫細胞在高風險和低風險 HCC 患者之間有差異浸潤。

結論：基於HMMR、MCM6、TPX2、KIF20A、CCL20、RGS2、NUSAP1、FABP5、FZD6、PBK和STK39的預後特徵有助於區分HCC患者的不同預後。

關鍵詞：乙型肝炎病毒（HBV）； 肝細胞癌 (HCC)； 總生存期； 預後簽名。

StephenW

Construction and validation of prognostic signature for hepatocellular carcinoma basing on hepatitis B virus related specific genes
Lei Wang #  1   2 , Manman Qiu #  3 , Lili Wu  4 , Zexing Li  5 , Xinyi Meng  6 , Lu He  7 , Bing Yang  8
Affiliations
Affiliations

    1
    Tianjin Second People's Hospital, Tianjin, 300192, China.
    2
    Tianjin Institute of Hepatology, Tianjin, 300192, China.
    3
    College of Life Sciences, Nankai University, Tianjin, 300071, China.
    4
    Logistics University of People's Armed Police Force, Tianjin, 300000, China.
    5
    School of Life Sciences, Tianjin University, Tianjin, 300072, China.
    6
    Department of Cell Biolopgy, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, 300070, China.
    7
    Department of Anatomy and Histology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, 300070, China.
    8
    Department of Cell Biolopgy, School of Basic Medical Sciences, Tianjin Medical University, Tianjin, 300070, China. [email protected].

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Contributed equally.

    PMID: 36474267 DOI: 10.1186/s13027-022-00470-y

Abstract

Background: Hepatocellular carcinoma (HCC) is a frequent primary liver cancer, and it is one of the leading cause of cancer-related deaths. Hepatitis B virus (HBV) infection is a crucial risk factor for HCC. Thus, this study aimed to explore the prognostic role of HBV-positive HCC related specific genes in HCC.

Methods: The HCC related data were downloaded from three databases, including The Cancer Genome Atlas (TCGA), International Cancer Genome Consortium (ICGC), and Gene Expression Omnibus (GEO). Univariate Cox regression analysis and LASSO Cox regression analysis were conducted to build the Risk score. Multivariate Cox regression analysis and survival analysis determined the independent prognostic indicators.

Results: After cross analysis of differentially expressed genes (DEGs), we have identified 106 overlapped DEGs, which were probably HBV-positive HCC related specific genes. These 106 DEGs were significantly enriched in 213 GO terms and 8 KEGG pathways. Among that, 11 optimal genes were selected to build a Risk score, and Risk score was an independent prognostic factor for HCC. High risk HCC patients had worse OS. Moreover, five kinds of immune cells were differentially infiltrated between high and low risk HCC patients.

Conclusion: The prognostic signature, based on HMMR, MCM6, TPX2, KIF20A, CCL20, RGS2, NUSAP1, FABP5, FZD6, PBK, and STK39, is conducive to distinguish different prognosis of HCC patients.

Keywords: Hepatitis B virus (HBV); Hepatocellular carcinoma (HCC); Overall survival; Prognostic signature.

StephenW

https://doi.org/10.1186/s13027-022-00470-y