进入受体和病毒标志物的存在表明，在一部分感染慢性乙型

StephenW

进入受体和病毒标志物的存在表明，在一部分感染慢性乙型肝炎的孕妇中，乙型肝炎病毒的胎盘复制水平较低

    Garima Garg、M. N. Meenu、Kajal Patel、Ravinder Singh、Priyal Gupta、Shashank Purwar、Sramana Mukhopadhyay、Nitu Mishra、Sudheer Gupta、Sumit Kumar Rawat、Harsh Goel、Rahul Kumar、Pranay Tanwar、Jitendra Singh、Shashwati Nema、Debasis Biswas、Nirupma Trehanpati、Anirudh K. Singh 和 Ashish Kumar Vyas

科学报告第 12 卷，文章编号：17795 (2022) 引用这篇文章

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抽象的

十多年来，人们已经知道乙型肝炎病毒 (HBV) 的经胎盘垂直传播途径。在这里，我们提出的证据表明 HBV 可以在胎盘中复制。在获得知情同意后，41 名 HBsAg 阳性和 10 名对照孕妇参加了研究。根据 2018 年 INASL 指南，HBV 阳性进一步分为高病毒载量 (HVL) 组和低病毒载量 (LVL) 组。通过 qPCR/RT-qPCR 和/ 或免疫组织化学 (IHC)。使用数字液滴 PCR 评估 cccDNA 的存在，而通过 qRT-PCR 和测序评估前基因组 (pg) RNA 的存在。通过 IHC 评估胎盘中 HBeAg 和 HBcAg 的存在。滋养细胞上 NTCP、HBeAg 和 HBcAg 的免疫染色以及总 HBV DNA、cccDNA 和 pgRNA 的存在表明，这些细胞不仅易受 HBV 感染，而且可能支持病毒复制。几个 HBeAg 血清学阴性样品的滋养细胞携带 HBeAg 的发现进一步支持了这一点。虽然，我们没有发现 NTCP 表达和病毒标记与病毒载量有任何相关性表明胎盘复制可能不会模仿肝细胞。在没有循环 HBeAg 的 HBV 感染女性的胎盘中存在 HBV 受体 NTCP 以及 cccDNA、pgRNA 和 HBeAg 表明胎盘充当复制宿主。

StephenW

Presence of entry receptors and viral markers suggest a low level of placental replication of hepatitis B virus in a proportion of pregnant women infected with chronic hepatitis B

    Garima Garg, M. N. Meenu, Kajal Patel, Ravinder Singh, Priyal Gupta, Shashank Purwar, Sramana Mukhopadhyay, Nitu Mishra, Sudheer Gupta, Sumit Kumar Rawat, Harsh Goel, Rahul Kumar, Pranay Tanwar, Jitendra Singh, Shashwati Nema, Debasis Biswas, Nirupma Trehanpati, Anirudh K. Singh & Ashish Kumar Vyas

Scientific Reports volume 12, Article number: 17795 (2022) Cite this article

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Abstract

The transplacental route of vertical transmission of Hepatitis B Virus (HBV) has been known for over a decade. Here we present evidence which suggest HBV can replicate in placenta. Forty-one HBsAg positive and 10 control pregnant women were enrolled in the study after obtaining informed consent. HBV positives were further divided in the High Viral Load (HVL) Group and Low Viral Load (LVL) Group according to INASL guidelines 2018. The Presence of the HBV DNA and expression of NTCP in the placenta was analyzed by qPCR/RT-qPCR and/or immunohistochemistry (IHC). The presence of cccDNA was assessed using Digital Droplet PCR while the presence of pre-genomic (pg) RNA was assessed through qRT-PCR and sequencing. The presence of HBeAg and HBcAg in the placenta was assessed by IHC. Immunostaining of NTCP, HBeAg and HBcAg on trophoblasts along with the presence of total HBV DNA, cccDNA and pgRNA indicated, that these cells are not only susceptible to HBV infection but may also support viral replication. This is further supported by the finding that trophoblasts of the several HBeAg seronegative samples harbored the HBeAg. Although, we did not find any correlation in NTCP expression and viral markers with viral load indicates placental replication may not aping hepatocytes. The presence of the HBV receptor, NTCP along with the presence of cccDNA, pgRNA, and HBeAg in placenta of HBV infected females without circulating HBeAg suggest that placenta act as a replication host.

StephenW

https://www.nature.com/articles/s41598-022-22699-8.pdf

乙肝人1949

可以。就是没有看明白，啥时候能给出时间