血清乙型肝炎病毒大中型表面蛋白作为预测 HBsAg 清除的新工

StephenW

血清乙型肝炎病毒大中型表面蛋白作为预测 HBsAg 清除的新工具
小琳 1 , 郑艳红 1 , 洪丽 2 , 路俊峰 1 , 单仁 1 , 刘一思 1 , 王晓晓 1 , 郑素军 1 , 马丽娜 1 , 曹振焕 2 , 陈心月 1
隶属关系
隶属关系

    1
    【作者单位】： 首都医科大学北京佑安医院肝病中心一科;
    2
    【作者单位】： 首都医科大学北京佑安医院肝病中心三科;

    PMID：36211341 PMCID：PMC9537546 DOI：10.3389/fimmu.2022.1028921

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背景：仍然缺乏可靠的乙型肝炎表面抗原 (HBsAg) 清除预测因子。最近的研究表明，大（LHBs）和中等乙型肝炎表面蛋白（MHBs）的水平与抗病毒效果密切相关。本研究旨在调查 LHB 和 MHB 水平预测 HBsAg 清除的可能性。

方法：将接受聚乙二醇干扰素（Peg-IFN）治疗的非活动性HBsAg携带者（IHC）队列根据HBsAg是否清除分为HBsAg清除组（R组）和HBsAg未清除组（NR组）。在 96 周。我们检测了 LHBs 和 MHBs 的水平，以评估预测 HBsAg 清除率的可能性。

结果：R组39例，NR组21例。 R 组基线和 12 周时的总 HBsAg、LHB 和 MHB 水平显着低于 NR 组（均 p<0.05）。多变量逻辑回归表明，基线和 12 周时的 LHB 和 MHB 水平是 HBsAg 清除的独立预测因子（OR = 0.435，p = 0.016；OR = 0.136，p = 0.003；OR = 0.137，p = 0.033；OR = 0.049，p = 0.043)。基线和 12 周 LHB 和 MHB 水平的曲线下面积（AUC）为 0.827-0.896，大于基线和 12 周总 HBsAg 水平（AUC：0.654-0.755）。与单一指标的预测结果相比，LHB和MHB水平联合预测HBsAg清除率具有更好的价值。从基线 LHB 和 MHB 构建的组合因子 1 和从 12 周 LHB 和 MHB 构建的组合因子 2 的 AUC 分别为 0.922 和 0.939，敏感性（82.05%-100.00%）和特异性（85.71%） -100.00%) 都很高。基于基线 LHBs ≤ 13.99 ng/mL 和 MHBs ≤ 7.95 ng/mL 的综合指标预测 HBsAg 清除率超过 90%。

结论：基线和 12 周 LHB 和 MHB 水平可以预测 IHC 中 Peg-IFN 治疗获得的 HBsAg 清除率，且预测值高于 HBsAg 总水平。

关键词：HBsAg清除； LHB； MHB；非活动性 HBsAg 携带者；聚乙二醇干扰素；预测器。

版权所有 © 2022 Lin, Zheng, Li, Lu, Ren, Liu, Wang, Zheng, Ma, Cao and Chen.
利益冲突声明

作者声明，该研究是在没有任何可能被解释为潜在利益冲突的商业或财务关系的情况下进行的。处理编辑 WY 在审查期间宣布与作者共享父母关系。

StephenW

Serum hepatitis B virus large and medium surface proteins as novel tools for predicting HBsAg clearance
Xiao Lin  1 , Yanhong Zheng  1 , Hong Li  2 , Junfeng Lu  1 , Shan Ren  1 , Yisi Liu  1 , Xiaoxiao Wang  1 , Sujun Zheng  1 , Lina Ma  1 , Zhenhuan Cao  2 , Xinyue Chen  1
Affiliations
Affiliations

    1
    First Department of Liver Disease Center, Beijing Youan Hospital, Capital Medical University, Beijing, China.
    2
    Third Department of Liver Disease Center, Beijing Youan Hospital, Capital Medical University, Beijing, China.

    PMID: 36211341 PMCID: PMC9537546 DOI: 10.3389/fimmu.2022.1028921

Free PMC article
Abstract

Background: There is still lack of reliable predictors for hepatitis B surface antigen (HBsAg) clearance. Recent studies have shown that the levels of large (LHBs) and medium hepatitis B surface proteins (MHBs) are closely related to antiviral efficacy. This study aimed to investigate the possibility of LHB and MHB levels to predict HBsAg clearance.

Methods: An inactive HBsAg carriers (IHCs) cohort that had received pegylated interferon (Peg-IFN) treatment was divided into the HBsAg-cleared group (R group) and the HBsAg non-cleared group (NR group) based on whether HBsAg was cleared at 96 weeks. We detected the levels of LHBs and MHBs to evaluate the possibility of predicting HBsAg clearance.

Results: There were 39 patients in the R group and 21 in the NR group. The total HBsAg, LHB, and MHB levels at baseline and at 12 weeks were significantly lower in the R group than in the NR group (all p< 0.05). Multivariate logistic regression indicated that LHB and MHB levels at baseline and 12 weeks were independent predictors of HBsAg clearance (OR = 0.435, p = 0.016; OR = 0.136, p = 0.003; OR = 0.137, p = 0.033; OR = 0.049, p = 0.043). The area under the curve (AUC) for the baseline and 12-week LHB and MHB levels was 0.827-0.896, which were greater than that of the total HBsAg level at baseline and 12-week (AUC: 0.654-0.755). Compared with the prediction results of a single indicator, the combination of LHB and MHB levels had better value in predicting HBsAg clearance. The AUCs of combination factor 1, constructed from baseline LHB and MHB, and combination factor 2, constructed from 12-week LHB and MHB, were 0.922 and 0.939, respectively, and the sensitivity (82.05%-100.00%) and specificity (85.71%-100.00%) were both high. The combined indicators based on baseline LHBs ≤ 13.99 ng/mL and MHBs ≤ 7.95 ng/mL predicted HBsAg clearance rate of more than 90%.

Conclusion: Baseline and 12-week LHB and MHB levels can predict HBsAg clearance obtained by Peg-IFN therapy in IHCs, and the predictive value is higher than that of the total HBsAg levels.

Keywords: HBsAg clearance; LHBs; MHBs; inactive HBsAg carriers; pegylated interferon; predictor.

Copyright © 2022 Lin, Zheng, Li, Lu, Ren, Liu, Wang, Zheng, Ma, Cao and Chen.
Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The handling editor WY declared a shared parent affiliation with the authors during the review.

StephenW

https://www.frontiersin.org/arti ... mu.2022.1028921/pdf