急性和慢性乙型肝炎感染患者的細胞因子譜

StephenW

急性和慢性乙型肝炎感染患者的細胞因子譜
Camilla Rodrigues de Almeida Ribeiro 1 , Daniela Gois Beghini 2 , Andreza Salvio Lemos 1 , Katrini Guidolini Martinelli 3 , Vinícius da Motta de Mello 4 , Nathalia Alves Araújo de Almeida 1 , Lia Laura Lewis-Ximeneza Sale 14
隸屬關係
隸屬關係

    1
    分子病毒學實驗室，Oswaldo Cruz 研究所，Oswaldo Cruz 基金會，巴西里約熱內盧。
    2
    巴西里約熱內盧奧斯瓦爾多克魯茲基金會奧斯瓦爾多克魯茲研究所治療、教學和生物產品創新實驗室。
    3
    巴西聖埃斯皮里圖聯邦大學社會醫學系。
    4
    病毒性肝炎實驗室，Oswaldo Cruz 研究所，Oswaldo Cruz 基金會，巴西里約熱內盧。

    PMID：34647645 DOI：10.1111/1348-0421.12947

抽象的

乙型肝炎病毒 (HBV) 是導致嚴重公共衛生威脅的急性和慢性肝炎的主要原因之一。眾所周知，細胞因子是調節免疫細胞分化、增殖和功能的重要化學介質，越來越多的證據表明免疫反應不足是導致 HBV 消除或持續存在的原因。該研究旨在確定 HBV 感染臨床過程中的細胞因子譜（IFN-γ、TNF-α、IL-2、IL-4、IL-6、IL-10 和 IL-17A）並研究與基因型的關聯.共有 66 份血漿樣本，其中 19 份來自急性乙型肝炎感染患者，47 份來自慢性乙型肝炎感染患者，通過生化測試、巢式 PCR、實時 PCR 和細胞因子進行了測試，使用商用 BD CBA 人 Th1/Th2/Th17 細胞因子試劑盒進行了評估。健康對照（10 名受試者）選自無肝病史的獻血者。在分析的基因型、病毒載量和細胞因子之間沒有發現相關性。與對照組相比，所有細胞因子在受感染個體中均顯示出更高水平的產生。通過 Spearman 相關係數發現細胞因子、IFN-Y、TNF、IL-10、IL-6、IL4 和 IL-2 之間存在中等至強的正相關。與慢性組和對照組相比，急性個體的 TNF (p = 0.009) IL-10 (p <0.001) 和 IL-6 (p <0.001) 更高，這些細胞因子可能參與病毒清除和預防慢性病。本文受版權保護。版權所有。

關鍵詞：細胞因子；乙型肝炎;免疫反應。

本文受版權保護。版權所有。

StephenW

CYTOKINES PROFILE IN PATIENTS WITH ACUTE AND CHRONIC HEPATITIS B INFECTION
Camilla Rodrigues de Almeida Ribeiro  1 , Daniela Gois Beghini  2 , Andreza Salvio Lemos  1 , Katrini Guidolini Martinelli  3 , Vinícius da Motta de Mello  4 , Nathalia Alves Araújo de Almeida  1 , Lia Laura Lewis-Ximenez  4 , Vanessa Salete de Paula  1
Affiliations
Affiliations

    1
    Laboratory of Molecular Virology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
    2
    Laboratory of Innovations in Therapies, Teaching and Bioproducts, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
    3
    Department of Social Medicine, Espírito Santo Federal University, Espírito Santo, Brazil.
    4
    Laboratory of Viral Hepatitis, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.

    PMID: 34647645 DOI: 10.1111/1348-0421.12947

Abstract

The hepatitis B virus (HBV) is one of the leading causes of acute and chronic hepatitis representing a serious public health threat. Cytokines are known to be important chemical mediators that regulate the differentiation, proliferation and function of immune cells and accumulating evidence indicate that the inadequate immune responses are responsible for HBV elimination or persistency. The study aimed to determine the cytokine profiles (IFN-γ, TNF-α, IL-2, IL-4, IL-6, IL-10 and IL-17A) at the clinical courses of HBV infection and investigate the association with genotypes. A total of 66 plasma samples, 19 from patients with acute and 47 with chronic hepatitis B infection were tested by biochemical tests, nested-PCR, real-time PCR and cytokines were evaluated using a commercial BD CBA Human Th1/Th2/Th17cytokines kit. Healthy controls (10 subjects) were selected from blood donors with no history of liver diseases. No correlation was found between genotypes, viral load and cytokines analyzed. All cytokines showed higher levels of production among infected individuals when compared to the control group. A positive correlation classified as moderate to strong was found between cytokines, IFN-Y, TNF, IL-10, IL-6, IL4 e IL-2 through Spearman's correlation coefficient. TNF (p = 0.009) IL-10 (p <0.001) and IL-6 (p <0.001) were higher in acute individuals compared to chronic and control group, theses cytokines could be involved in viral elimination and protection against chronicity. This article is protected by copyright. All rights reserved.

Keywords: Cytokines; Hepatitis B; Immune response.

This article is protected by copyright. All rights reserved.