CRISPR/Cas9系统在乙型肝炎肝癌治疗中的应用

StephenW

CRISPR/Cas9系统在乙型肝炎肝癌治疗中的应用
魏吕1、桃李1、王珊珊1、王慧慧1、李雪梅1、张树兵1、王莲子1、徐元红1、魏伟1
隶属关系
联系

    1
    36639安徽医科大学第一附属医院，中国 合肥

    PMID：34605326 DOI：10.1177/15330338211045206

抽象的

成簇的规则间隔短回文重复序列 (CRISPR)/CRISPR 相关蛋白 9 (Cas9) 系统最初是在原核生物中发现的，并作为适应性免疫系统的一部分发挥作用。许多学者的实验研究以及科学技术的进步使原核生物衍生的 CRISPR/Cas 基因组编辑系统改变了我们操纵、检测、成像和注释活细胞中特定 DNA 和 RNA 序列的能力。多样的物种。通过现代基因工程编辑技术和高通量基因测序，我们可以编辑和剪接共价闭合环状DNA使其沉默，纠正肝癌基因的突变和缺失，实现缺陷基因的精确原位修复，防止病毒感染或复制。这种操作不会破坏整个基因组的结构，并有助于疾病的治愈。在这篇综述中，我们讨论了 CRISPR/Cas 可用于治疗乙型肝炎病毒感染引起的肝癌患者的可能性，并回顾了这种有效的基因编辑技术所带来的挑战。

关键词：CRISPR/Cas9系统；基因编辑；乙型肝炎病毒DNA；肝癌

StephenW

The Application of the CRISPR/Cas9 System in the Treatment of Hepatitis B Liver Cancer
Wei Lv  1 , Tao Li  1 , Shanshan Wang  1 , Huihui Wang  1 , Xuemei Li  1 , Shubing Zhang  1 , Lianzi Wang  1 , Yuanhong Xu  1 , Wei Wei  1
Affiliations
Affiliation

    1
    36639The First Affiliated Hospital of Anhui Medical University, Hefei, China.

    PMID: 34605326 DOI: 10.1177/15330338211045206

Abstract

The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system was originally discovered in prokaryotes and functions as part of the adaptive immune system. The experimental research of many scholars, as well as scientific and technological advancements, has allowed prokaryote-derived CRISPR/Cas genome-editing systems to transform our ability to manipulate, detect, image, and annotate specific DNA and RNA sequences in the living cells of diverse species. Through modern genetic engineering editing technology and high-throughput gene sequencing, we can edit and splice covalently closed circular DNA to silence it, and correct the mutation and deletion of liver cancer genes to achieve precise in situ repair of defective genes and prohibit viral infection or replication. Such manipulations do not destroy the structure of the entire genome and facilitate the cure of diseases. In this review, we discussed the possibility that CRISPR/Cas could be used as a treatment for patients with liver cancer caused by hepatitis B virus infection, and reviewed the challenges incurred by this effective gene-editing technology.

Keywords: CRISPR/Cas9 system; gene editing; hepatitis B virus DNA; liver cancer.