C8B在补体和凝血级联信号通路中是肝细胞癌患者生存的预测

StephenW

C8B in Complement and Coagulation Cascades Signaling Pathway is a predictor for Survival in Hepatocellular Carcinoma Patients


Authors Zhang Y, Chen X, Cao Y, Yang Z

Received 20 January 2021

Accepted for publication 25 March 2021

Published 22 April 2021 Volume 2021:13 Pages 3503—3515

DOI https://doi.org/10.2147/CMAR.S302917

Checked for plagiarism Yes

Review by Single anonymous peer review

Peer reviewer comments 2

Editor who approved publication: Dr Eileen O'Reilly
Yuan Zhang,1 Xiaorong Chen,1 Yajuan Cao,2 Zongguo Yang1

1Department of Integrative Medicine, Shanghai Public Health Clinical Center, Fudan University, Shanghai, 201508, People’s Republic of China; 2Clinical Translation Research Center, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, 200433, People’s Republic of China

Correspondence: Zongguo Yang
Department of Integrative Medicine, Shanghai Public Health Clinical Center, Fudan University, 2901 Caolang Road, Shanghai, 201508, People’s Republic of China
Email [email protected]
Yajuan Cao
Clinical Translation Research Center, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, 200433, People’s Republic of China
Email [email protected]

Objective: The role of the complement and coagulation cascades signaling pathway in the pathogenesis of cancers remains uncertain. This study aimed to investigate the associations between enriched differentially expressed genes (DEGs) in this pathway and hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) patients.
Materials and Methods: Clinical and gene expression data of the Gene Expression Omnibus (GEO) series profile GSE14520 were downloaded. The “Limma” package was used to screen the DEGs and the “clusterProfiler” package was used to identify the complement and coagulation cascades pathway and enriched significant genes. Cox regression analysis, the Kaplan–Meier method, and the nomogram model were used to address the correlations between significantly enriched DEGs in the complement and coagulation cascades pathway and HCC survival.
Results: A total of 220 HBV-related HCC patients were enrolled in this study. The complement and coagulation cascades pathway was significantly enriched by 37 DEGs (p-value < 0.05 and adjusted p-value < 0.05). Complement 8 beta chain (C8B) expression levels had protective effects on overall survival (OS) and recurrence-free survival (RFS) in HBV-related HCC patients. High levels of C8B contributed to favorable OS and RFS in this population (both p < 0.01), even after adjustment of clinicopathological characteristics including tumor node metastasis (TNM) staging, Barcelona Clinic liver cancer (BCLC) staging, gender, and fibrinogen beta chain (FGB) expression (all p < 0.05).
Conclusion: C8B in the complement and coagulation cascades signaling pathway serves as a predictive candidate for survival in HBV-related HCC patients.

Keywords: C8B, FGB, complement and coagulation cascades, hepatocellular carcinoma, survival

StephenW

C8B在补体和凝血级联信号通路中是肝细胞癌患者生存的预测因子


作者张Y，陈X，曹Yang，杨Z

2021年1月20日收到

接受发表于2021年3月25日

2021年4月22日发布第2021：13卷第3503至3515页

DOI https://doi.org/10.2147/CMAR.S302917

检查抄袭是

由单个匿名同行评审进行评审

同行审稿人评论2

批准出版的编辑：Eileen O'Reilly博士
张元1陈晓荣1曹亚娟2杨宗国1

1复旦大学上海公共卫生临床中心中西医结合系，上海201508； 2同济大学医学院附属上海肺科医院临床翻译研究中心，上海200433

通讯：杨宗国
复旦大学上海公共卫生临床中心中西医结合系，上海市Ca浪路2901号，中国，201508
电子邮件[email protected]
曹亚娟
同济大学医学院附属上海肺科医院临床翻译研究中心，上海200433
电子邮件[email protected]

目的：补体和凝血级联信号通路在癌症发病机理中的作用尚不确定。这项研究旨在调查该途径中富集的差异表达基因（DEG）与乙型肝炎病毒（HBV）相关的肝细胞癌（HCC）患者之间的关联。
材料和方法：下载了Gene Expression Omnibus（GEO）系列概况GSE14520的临床和基因表达数据。 “ Limma”软件包用于筛选DEG，“ clusterProfiler”软件包用于鉴定补体和凝血级联途径并富集重要基因。使用Cox回归分析，Kaplan-Meier方法和列线图模型来解决补体和凝血级联途径中显着富集的DEG与HCC生存之间的相关性。
结果：本研究共纳入220名HBV相关的HCC患者。补体和凝血级联途径显着富集了37个DEs（p值<0.05和调整后的p值<0.05）。补体8β链（C8B）表达水平对HBV相关HCC患者的总生存期（OS）和无复发生存期（RFS）具有保护作用。即使在调整临床病理特征（包括肿瘤结转移（TNM）分期，巴塞罗那临床肝癌（BCLC）分期，性别和纤维蛋白原β链）后，高水平的C8B仍对该人群的OS和RFS有利（均p <0.01）。 （FGB）表达（所有p <0.05）。
结论：补体和凝血级联信号通路中的C8B可作为HBV相关HCC患者生存的预测候选者。

关键词：C8B，FGB，补体和凝血级联，肝细胞癌，生存

StephenW

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