健康成年人中针对乙型肝炎疫苗的抗体反应后，B和T细胞受体

StephenW

The dynamics and association of B and T cell receptor repertoires upon antibody response to hepatitis B vaccination in healthy adults
Miaoxian Zhao  1 , Xueying Li  1 , Shi Xie  1 , Mingxing Gong  1 , Rong Yan  1 , Anqi Zheng  1 , Ying Xu  1 , Hongkai Wu  2 , Zhanhui Wang  1
Affiliations
Affiliations

    1
    State Key Laboratory of Organ Failure Research, Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Department of Infectious Diseases and Hepatology Unit, Nanfang Hospital, Southern Medical University, Guangzhou, China.
    2
    State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, China.

    PMID: 33861159 DOI: 10.1080/21645515.2021.1913028

Abstract

The Hepatitis B (HB) vaccine is efficacious in preventing hepatitis B virus infection. However, the association between antibody response to the HB vaccine and dynamic immune repertoire changes in different cell subsets remains unclear. Nine healthy participants were administered three doses of HB vaccine following the 0, 1, 6 month schedule. Peripheral CD4+ T, memory B (MB), naïve B (NB), and plasma cells (PCs) were sorted before vaccination and 7 days following each dose. The complementary determining region 3 of T-cell receptor β (TCRβ) chain and B-cell receptor (BCR) heavy chain (IgG, IgM, IgA) repertoires were analyzed by high-throughput sequencing. All nine participants elicited protective antibody titers to the vaccine at the end of immunization. Compared with the baseline, MB cells showed a significant increase in IgG usage and decreased IgM usage and repertoire diversity at the end of vaccination. TCRβ diversity changes were highly correlated with those of the BCR in MB cells in participants with a faster and robust antibody responses. The percentage of shared clonotypes between NB and MB cells, and MB cells and PCs were much higher than that between NB cells and PCs. The more clonotypes sharing the faster and stronger antibody responses were observed after HB vaccination. These results suggest the integral involvement of MB cells in vaccine immunization. Interaction between CD4+ T and MB cells and B cell differentiation may improve antibody response to HB vaccine.

Keywords: B cell receptor; Hepatitis B vaccine; T cell receptor; antibody response; repertoire.

StephenW

健康成年人中针对乙型肝炎疫苗的抗体反应后，B和T细胞受体组成的动态变化和关联
赵妙贤1，李学英1，谢ie 1，龚明兴1，荣Yan 1，郑安琪1，徐颖1，吴洪凯2，王占辉1
隶属关系
隶属关系

    1个
    南方医科大学附属南方医院传染病与肝病科，器官衰竭研究国家重点实验室，广东省病毒性肝炎研究重点实验室，广州。
    2个
    广州医科大学附属第一医院，呼吸疾病国家重点实验室，国家呼吸疾病临床研究中心，广州，广州。

    PMID：33861159 DOI：10.1080 / 21645515.2021.1913028

抽象的

乙型肝炎（HB）疫苗可有效预防乙型肝炎病毒感染。然而，对HB疫苗的抗体反应与不同细胞亚群中动态免疫库变化之间的关联尚不清楚。按照0、1、6个月的时间表，对9名健康参与者进行了3剂HB疫苗接种。在接种疫苗之前和每次给药后7天，对外周CD4 + T，记忆B（MB），幼稚B（NB）和浆细胞（PC）进行分类。通过高通量测序分析了T细胞受体β（TCRβ）链和B细胞受体（BCR）重链（IgG，IgM，IgA）组成部分的互补决定区3。在免疫结束时，所有九名参与者都引发了疫苗的保护性抗体滴度。与基线相比，MB细胞在疫苗接种结束时显示IgG使用量显着增加，IgM使用量和库多样性降低。参与者的TCRβ多样性变化与MB细胞中BCR的变化高度相关，具有更快，更强的抗体反应。 NB和MB细胞之间，MB细胞和PC之间共享克隆型的百分比远高于NB细胞和PC之间的共享克隆型百分比。 HB疫苗接种后，观察到更多克隆型共享更快和更强的抗体反应。这些结果表明MB细胞参与疫苗免疫。 CD4 + T和MB细胞之间的相互作用以及B细胞分化可能会改善对HB疫苗的抗体反应。

关键字：B细胞受体；乙肝疫苗； T细胞受体；抗体反应；曲目