致编辑的信：HBV pgRNA的逆转录效率与HBV cccDNA的转录效率之间

StephenW

Letter to the Editor: The differences between the reverse transcriptional efficiency of HBV pgRNA and transcriptional efficiency of HBV cccDNA
Hao Liao  1 , Yan Liu  2 , Jun Wang  3 , Guanxun Cheng  1 , Dongping Xu  2 , Junhui Chen  1 , Fengmin Lu  4
Affiliations
Affiliations

    1
    Intervention and Cell Therapy Center, Peking University Shenzhen Hospital, Shenzhen Peking University, The Hong Kong University of Science and Technology Medical Center, Shenzhen, 518035, Guangdong province, China.
    2
    Department of Infectious Diseases, The Fifth Medical Center of Chinese PLA General Hospital, Beijing, 100039, China.
    3
    Peking University Ditan Teaching Hospital, Beijing, 100015, China.
    4
    Department of Microbiology & Infectious Disease Center, School of Basic Medical, Sciences, Peking University Health Science Center, Beijing, 100191, China.

    PMID: 33665865 DOI: 10.1002/hep.31786

Abstract

We read with great interest the article entitled "HBV RNA profiles in chronic hepatitis B patients under different disease phases and anti-viral therapy" authored by Mak et al(1). The authors proposed that the reverse transcription efficiency in HBeAg-positive patients was significantly higher than that in HBeAg-negative patients, based on the RNA: DNA ratio in the two groups of patients (0.79 vs. 0.53; p< 0.001). We had found that a higher ratio of serum HBV RNA to HBV DNA was inversely related with reverse transcription efficiency of HBV pgRNA(2), and HBeAg-positive patients had a lower reverse transcription efficiency of HBV pgRNA than HBeAg-negative patients did. We here suggest that the ratio of serum HBV DNA: (HBV DNA + HBV RNA) is more reliable to assess the reverse transcriptional efficiency of pgRNA. The medians of ratio in our cohort were 0.56 (n = 54), 0.56 (n = 23), 0.69 (n = 64) and 0.66 (n = 4), for patients with HBeAg-positive chronic infection, HBeAg-positive chronic hepatitis, HBeAg-negative chronic infection, and HBeAg-negative chronic hepatitis, respectively. Inconsistent with the report by Mak et al., our data showed that the reverse transcriptional efficiency was lower in HBeAg-positive patients than in HBeAg-negative patients (0.56 vs. 0.68; p< 0.001). A schematic diagram for illustrating the efficiencies of covalently closed circular DNA (cccDNA) transcription and reverse transcription of pgRNA are shown in Figure 1.

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StephenW

致编辑的信：HBV pgRNA的逆转录效率与HBV cccDNA的转录效率之间的差异
廖浩1，柳柳2，王军3，程冠勋1，徐东平2，陈俊辉1，卢凤民4
隶属关系
隶属关系

    1个
    北京大学深圳医院干预与细胞治疗中心，深圳大学，香港科技大学医学中心，广东深圳518035。
    2个
    中国人民解放军总医院第五医疗中心传染病科，北京100039
    3
    北京大学地坛教学医院，北京100015
    4
    北京大学健康科学中心基础医学院，微生物与传染病中心，北京100191

    PMID：33665865 DOI：10.1002 / hep.31786

抽象的

我们非常感兴趣地阅读了由Mak等（1）撰写的题为“慢性乙型肝炎患者在不同疾病阶段和抗病毒治疗下的HBV RNA谱”的文章。作者提出，基于两组患者的RNA：DNA比率，HBeAg阳性患者的逆转录效率显着高于HBeAg阴性患者（0.79 vs. 0.53； p <0.001）。我们发现，血清HBV RNA与HBV DNA的比例较高与HBV pgRNA的逆转录效率呈负相关（2），并且HBeAg阳性患者的HBV pgRNA的逆转录效率低于HBeAg阴性患者。我们在这里建议血清HBV DNA：（HBV DNA + HBV RNA）的比率对于评估pgRNA的逆转录效率更为可靠。对于HBeAg阳性慢性感染，HBeAg阳性慢性肝炎患者，我们队列的比率中位数分别为0.56（n = 54），0.56（n = 23），0.69（n = 64）和0.66（n = 4）。 ，HBeAg阴性慢性感染和HBeAg阴性慢性肝炎。与Mak等人的报告不一致，我们的数据表明，HBeAg阳性患者的逆转录效率低于HBeAg阴性患者（0.56比0.68； p <0.001）。图1显示了共价闭合环状DNA（cccDNA）转录和pgRNA逆转录效率的示意图。

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