正在进行的慢性乙型肝炎感染期间，分类的B细胞转录组指向

StephenW

Sorted B cell transcriptomes point towards actively regulated B cell responses during ongoing chronic hepatitis B infections
Stijn Van Hees  1 , Bart Cuypers  2 , Stefan Bourgeois  3 , Zwier M A Groothuismink  4 , Pieter Meysman  5 , Pieter Van der Vlies  6 , Rob de Knegt  4 , Luisa Vonghia  1 , Peter Michielsen  1 , Sven Francque  1 , Kris Laukens  5 , Andre Boonstra  4 , Thomas Vanwolleghem  7
Affiliations
Affiliations

    1
    Department of Gastroenterology and Hepatology, Antwerp University Hospital, Wilrijkstraat 10, 2650 Antwerp, Belgium; Laboratory of Experimental Medicine and Paediatrics, Antwerp University, Universiteitsplein 1, 2610 Antwerp, Belgium.
    2
    Department of Biomedical Sciences, Institute of Tropical Medicine, Kronenburgstraat 43, 2000 Antwerp, Belgium; Department of Mathematics and Computer Science, University of Antwerp, Middelheimlaan 1, 2020 Antwerp, Belgium.
    3
    Department of Gastroenterology and Hepatology, Antwerp University Hospital, Wilrijkstraat 10, 2650 Antwerp, Belgium; Department of Gastroenterology and Hepatology, ZNA Stuivenberg, Antwerp, Belgium.
    4
    Department of Gastroenterology and Hepatology, Erasmus Medical Center Rotterdam, the Netherlands.
    5
    Department of Mathematics and Computer Science, University of Antwerp, Middelheimlaan 1, 2020 Antwerp, Belgium.
    6
    Department of Genetics, University Medical Center Groningen, the Netherlands.
    7
    Department of Gastroenterology and Hepatology, Antwerp University Hospital, Wilrijkstraat 10, 2650 Antwerp, Belgium; Laboratory of Experimental Medicine and Paediatrics, Antwerp University, Universiteitsplein 1, 2610 Antwerp, Belgium; Department of Gastroenterology and Hepatology, Erasmus Medical Center Rotterdam, the Netherlands. Electronic address: [email protected].

    PMID: 33548734 DOI: 10.1016/j.cellimm.2021.104283

Abstract

The natural course of chronic hepatitis B virus (HBV) infections follows distinct clinical disease phases, characterized by fluctuating levels of serum HBV DNA and ALT. The immune cells and their features that govern these clinical disease transitions remain unknown. In the current study, we performed RNA sequencing on purified B cells from blood (n = 42) and liver (n = 10) of healthy controls and chronic HBV patients. We found distinct gene expression profiles between healthy controls and chronic HBV patients, as evidenced by 190 differentially expressed genes (DEG), but also between the clinical phenotypes of a chronic HBV infection (17-110 DEG between each phase). Numerous immune pathways, including the B cell receptor pathway were upregulated in liver B cells when compared to peripheral B cells. Further investigation of the detected DEG suggested an activation of B cells during HBeAg seroconversion and an active regulation of B cell signalling in the liver.

Keywords: Activation; B cells; HBeAg seroconversion; Hepatitis B; Intrahepatic; RNA sequencing.

Copyright © 2021 Elsevier Inc. All rights reserved.

StephenW

正在进行的慢性乙型肝炎感染期间，分类的B细胞转录组指向积极调节的B细胞反应
Stijn Van Hees 1，Bart Cuypers 2，Stefan Bourgeois 3，Zwier MA Groothuismink 4，Pieter Meysman 5，Pieter Van der Vlies 6，Rob de Knegt 4，Luisa Vonghia 1，Peter Michielsen 1，Sven Francque 1，Kris Laukens 5，Andre Boonstra 4，Thomas Vanwolleghem 7
隶属关系
隶属关系

    1个
    安特卫普大学医院消化内科和肝病科，比利时安特卫普2650 Wilrijkstraat 10；安特卫普大学实验医学和儿科学实验室，比利时安特卫普Universiteitsplein 1，2610。
    2
    热带医学研究所生物医学科学系，比利时安特卫普，Kronenburgstraat 43，2000；安特卫普大学数学和计算机科学系，比利时安德卫普，Middelheimlaan 1，2020。
    3
    安特卫普大学医院消化内科和肝病科，比利时安特卫普2650 Wilrijkstraat 10；比利时安特卫普ZNA Stuivenberg胃肠病学和肝病学系。
    4
    荷兰鹿特丹伊拉斯姆斯医学中心消化内科和肝病科。
    5
    安特卫普大学数学和计算机科学系，比利时安德卫普，Middelheimlaan 1，2020。
    6
    荷兰格罗宁根大学医学中心遗传学系。
    7
    安特卫普大学医院消化内科和肝病科，比利时安特卫普2650 Wilrijkstraat 10；安特卫普大学实验医学与儿科学实验室，比利时安特卫普Universiteitsplein 1，2610；荷兰鹿特丹伊拉斯姆斯医学中心消化内科和肝病科。电子地址：[email protected]。

    PMID：33548734 DOI：10.1016 / j.cellimm.2021.104283

抽象

慢性乙型肝炎病毒（HBV）感染的自然过程遵循不同的临床疾病阶段，其特征在于血清HBV DNA和ALT水平波动。免疫细胞及其控制这些临床疾病转变的特征仍然未知。在当前研究中，我们对健康对照和慢性HBV患者血液（n = 42）和肝脏（n = 10）的纯化B细胞进行了RNA测序。我们发现，在健康对照组和慢性HBV患者之间存在明显的基因表达谱，这由190个差异表达基因（DEG）证明，而且在慢性HBV感染的临床表型之间也是如此（每个阶段之间为17-110 DEG）。与外周血B细胞相比，肝B细胞中包括B细胞受体途径在内的许多免疫途径均被上调。对检测到的DEG的进一步研究表明，HBeAg血清转化过程中B细胞活化，肝脏中B细胞信号传导活跃。

关键词：激活B细胞； HBeAg血清转化；乙型肝炎；肝内RNA测序。

版权所有©2021 Elsevier Inc.保留所有权利。