靶向乙型肝炎病毒聚合酶的单克隆抗体的制备和功能评价

StephenW

Preparation and functional evaluation of monoclonal antibodies targeting Hepatitis B Virus Polymerase
Song Hu  1 , Hualong Xiong  2 , Xiaozhen Kang  2 , Shaojuan Wang  2 , Tianying Zhang  2 , Quan Yuan  2 , Deying Tian  1
Affiliations
Affiliations

    1
    Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology , Wuhan 430030, Hubei Province, China.
    2
    School of Life Science & School of Public Health; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics; National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Xiamen University , Xiamen 361102, China.

    PMID: 33356842 DOI: 10.1080/21505594.2020.1869391

Abstract

Background: HBV pol plays a critical role in the replication of hepatitis B virus (HBV). Previous studies conducted on HBV pol have produced limited evidence on HBV pol expression due to the lack of effective detection methods.

Methods: The present study used the HBV pol (159-406 aa) protein as a target to screen for specific monoclonal antibodies that recognize HBV pol and subsequently evaluate their diagnostic and therapeutic value.

Results: Four antibodies (P3, P5, P12, P20) against HBV pol were obtained. Among them, the P20 antibody indicated optimal binding with HBV pol as demonstrated by Western blotting (WB) in a cell model transfected with the HBV genome. We also expressed P5 and P12 antibodies in mouse liver cells by transfection and the results indicated significant antiviral effects caused by these two antibodies especially P12.

Conclusion: In summary, the present study established an antibody which was denoted P20. This antibody can be used to detect HBV pol expression by four HBV genomes via WB analysis. In addition, the antibody denoted P12 could exert antiviral effects via intracellular expression, which may provide a promising approach for the treatment of chronic hepatitis B.

Keywords: HBV; antibody; detection; polymerase; therapy.

StephenW

靶向乙型肝炎病毒聚合酶的单克隆抗体的制备和功能评价
宋虎1，熊华龙2，康小珍2，王少娟2，张天颖2，全院2，田德英1
隶属关系
隶属关系

    1个
    华中科技大学同济医学院附属同济医院，湖北武汉430030
    2
    生命科学学院和公共卫生学院；分子疫苗与分子诊断国家重点实验室厦门大学国家传染病诊断与疫苗研究所，厦门361102

    PMID：33356842 DOI：10.1080 / 21505594.2020.1869391

抽象

背景：HBV pol在乙型肝炎病毒（HBV）复制中起关键作用。由于缺乏有效的检测方法，先前对HBV pol进行的研究仅获得了关于HBV pol表达的有限证据。

方法：本研究以HBV pol（159-406 aa）蛋白为靶标，筛选可识别HBV pol的特异性单克隆抗体，随后评估其诊断和治疗价值。

结果：获得了针对HBV pol的四种抗体（P3，P5，P12，P20）。其中，P20抗体表明与HBV pol的最佳结合，如Western blotting（WB）在转染HBV基因组的细胞模型中所证实的。我们还通过转染在小鼠肝细胞中表达了P5和P12抗体，结果表明这两种抗体尤其是P12引起了显着的抗病毒作用。

结论：总之，本研究建立了一种抗体，命名为P20。该抗体可通过WB分析用于检测四个HBV基因组的HBV pol表达。此外，P12抗体可通过细胞内表达发挥抗病毒作用，这可能为慢性乙型肝炎的治疗提供了一种有希望的方法。

关键字：HBV；抗体;检测;聚合酶治疗。

StephenW

https://www.tandfonline.com/doi/ ... 391?needAccess=true