循环中的滤泡性树突状细胞扩大，可促进慢性HBV感染的免疫

StephenW

Expanded circulating follicular dendritic cells facilitate immune responses in chronic HBV infection
Xiaoyi Li  1 , Qifan Zhang  2 , Wanyue Zhang  3 , Guofu Ye  1 , Yanchen Ma  1 , Chunhua Wen  1 , Shuqin Gu  1 , Libo Tang  1 , Yongyin Li  4
Affiliations
Affiliations

    1
    State Key Laboratory of Organ Failure Research, Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, No. 1838 North Guangzhou Avenue, Guangzhou, 510515, China.
    2
    Division of Hepatobiliopancreatic Surgery, Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China.
    3
    The Air Force Hospital of Southern Theater Command, Guangzhou, China.
    4
    State Key Laboratory of Organ Failure Research, Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, No. 1838 North Guangzhou Avenue, Guangzhou, 510515, China. [email protected].

    PMID: 33160362 DOI: 10.1186/s12967-020-02584-6

Abstract

Background: The restoration of host hepatitis B virus (HBV)-specific antiviral immunity is an effective strategy for hepatitis B recovery. Follicular dendritic cells (FDCs) play a crucial role in immune regulation. The goal of the present study was to investigate the characteristics and functions of FDCs in chronic HBV infection.

Methods: The frequencies of FDCs in peripheral blood, liver, and spleen were measured in patients with chronic HBV infection. Isolated FDCs from splenic tissues of HBV-related liver cirrhosis-induced hypersplenism patients were cultured with autologous intrasplenic CD4+ T cells and CD19+ B cells.

Results: We observed that patients with chronic HBV infection had a significantly increased frequency of circulating FDCs compared to that of healthy controls. Additionally, the frequency of circulating FDCs was positively correlated with that of intrahepatic and intrasplenic counterparts. Moreover, positive correlations were observed between the frequencies of circulating FDCs and plasmablast and memory B cells, as well as C-X-C motif chemokine receptor type 5 (CXCR5)+CD4+ T cells and CXCR5+CD8+ T cells. Notably, in vitro experimental results demonstrated that FDCs derived from splenic tissues of chronic HBV patients facilitated interferon-γ and interleukin-21 production from autologous intrasplenic CD4+ T cells and promoted the proliferation of autologous intrasplenic CD19+ B cells.

Conclusions: Expanded FDCs in patients with chronic HBV infection may favor host immune responses against HBV. The identification of this unique population of cell may contribute to a better understanding of the immune regulatory mechanisms associated with chronic HBV infection and provide a potential immunotherapeutic target for this disease.

Keywords: B cells; Follicular dendritic cells; Hepatitis B virus; T cells.

StephenW

循环中的滤泡性树突状细胞扩大，可促进慢性HBV感染的免疫反应
李晓怡1，张启帆2，张万跃3，叶国富1，马艳晨1，温春华1，谷书琴1，唐立波1，李永银4
隶属关系
隶属关系

    1个
    南方医科大学附属南方医院传染病科，广东省病毒性肝炎研究重点实验室，器官衰竭研究国家重点实验室，广州市广州北大街1838号，广东广州510515。
    2
    南方医科大学附属南方医院肝胆胰外科，普外科。
    3
    南方战区司令部空军医院，广州。
    4
    南方医科大学附属南方医院传染病科，广东省病毒性肝炎研究重点实验室，器官衰竭研究国家重点实验室，广州市广州北大街1838号，广东广州510515。 [email protected]。

    PMID：33160362 DOI：10.1186 / s12967-020-02584-6

抽象

背景：恢复宿主乙型肝炎病毒（HBV）特有的抗病毒免疫力是乙型肝炎康复的有效策略。滤泡树突状细胞（FDC）在免疫调节中起关键作用。本研究的目的是调查FDC在慢性HBV感染中的特征和功能。

方法：测定慢性HBV感染患者外周血，肝脏和脾脏中FDC的频率。用自体脾内CD4 + T细胞和CD19 + B细胞培养从HBV相关肝硬化引起的脾功能亢进患者脾脏组织分离的FDC。

结果：我们观察到，与健康对照组相比，慢性HBV感染患者的FDC循环频率显着增加。此外，循环的FDC的频率与肝内和脾内对应物的频率呈正相关。而且，观察到循环的FDC与浆母细胞和记忆B细胞的频率以及C-X-C基序趋化因子受体5型（CXCR5）+ CD4 + T细胞和CXCR5 + CD8 + T细胞之间存在正相关。值得注意的是，体外实验结果表明，源自慢性HBV患者脾脏组织的FDC促进了自体脾脏CD4 + T细胞的干扰素-γ和白介素21的产生，并促进了自体脾脏CD19 + B细胞的增殖。

结论：慢性HBV感染患者的FDCs扩大可能有利于宿主对HBV的免疫反应。这种独特的细胞群的鉴定可能有助于更好地了解与慢性HBV感染相关的免疫调节机制，并为该疾病提供潜在的免疫治疗靶点。

关键词：B细胞；滤泡树突状细胞；乙型肝炎病毒; T细胞。

StephenW

https://translational-medicine.b ... /s12967-020-02584-6