HBx作為一種癌基因，可在體內和體外促進肝細胞癌的侵襲和

StephenW

HBx acts as an oncogene and promotes the invasion and metastasis of hepatocellular carcinoma both in vivo and vitro
Shizhong Yang  1 , Yanfeng Liu  2 , Xiaobin Feng  1 , Xiaojuan Wang  1 , Meilong Wu  1 , Lei Gong  1 , Bin Shu  1 , Qian Lu  1 , Jiahong Dong  3
Affiliations
Affiliations

    1
    Hepato-Pancreato-Biliary Center, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing, China.
    2
    Department of Hepato-Biliary Surgery, Qilu Hospital of Shandong University, Jinan, China.
    3
    Hepato-Pancreato-Biliary Center, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing, China. Electronic address: [email protected].

    PMID: 33153927 DOI: 10.1016/j.dld.2020.10.007

Abstract

Background and aims: Hepatitis B virus X (HBx) has been reported to be closely related to hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC). This study aimed to detect the expression pattern of HBx and explore whether HBx protein can promote HCC invasion and metastasis both in vivo and vitro.

Methods: HBx expression was detected in HCC tissues via immunochemistry. A recombinant adenovirus vector containing the HBx gene was constructed and transfected into the HCC cell line SMMC-7721. Wound healing, transwell migration, and invasion assays were performed to evaluate migration and invasion potentials. A splenic implant tumor nude mice model was established to confirm its invasion and metastatic abilities in vivo.

Results: The positive rate of HBx in HCC tissues was 67.89%. HBx overexpression significantly promoted the migration and invasion abilities of SMMC-7721 cells in vitro. The tumor model showed that splenic implant tumor volume and number of liver metastatic tumor nodes were significantly larger and higher in the HBx overexpression group than in the control group.

Conclusions: HBx is highly expressed in HCC tissues and promotes HCC invasion and metastasis both in vivo and vitro with oncogene activity, thereby suggesting that HBx can serve as a novel therapeutic target in HCC.

Keywords: Hepatitis B virus X; Hepatocellular carcinoma; Invasion; Metastasis.

Copyright © 2020 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd. All rights reserved.

StephenW

HBx作為一種癌基因，可在體內和體外促進肝細胞癌的侵襲和轉移
楊世忠1，劉彥峰2，馮小斌1，王小娟1，吳梅龍1，雷公1，彬書1，前路1，嘉鴻洞3
隸屬關係
隸屬關係

    1個
    清華大學臨床醫學院，北京清華長庚醫院肝胰膽管中心，北京。
    2
    山東大學齊魯醫院肝膽外科，濟南
    3
    清華大學臨床醫學院，北京清華長庚醫院肝胰膽管中心，北京。電子地址：[email protected]。

    PMID：33153927 DOI：10.1016 / j.dld.2020.10.007

抽象

背景與目的：據報導，乙型肝炎病毒X（HBx）與乙型肝炎病毒（HBV）相關的肝細胞癌（HCC）密切相關。這項研究旨在檢測HBx的表達模式，並探討HBx蛋白是否可以在體內和體外促進HCC的侵襲和轉移。

方法：採用免疫化學方法在肝癌組織中檢測HBx的表達。構建了含有HBx基因的重組腺病毒載體，並將其轉染到HCC細胞系SMMC-7721中。進行傷口癒合，跨孔遷移和侵襲分析以評估遷移和侵襲潛力。建立脾植入腫瘤裸鼠模型以證實其在體內的侵襲和轉移能力。

結果：HCC組織中HBx陽性率為67.89％。 HBx過表達顯著促進SMMC-7721細胞的體外遷移和侵襲能力。腫瘤模型顯示，HBx過表達組的脾臟植入物腫瘤體積和肝轉移性腫瘤結點數量明顯多於對照組。

結論：HBx在肝癌組織中高表達，通過癌基因活性在體內外促進肝癌的侵襲和轉移，提示HBx可作為肝癌的新型治療靶點。

關鍵詞：乙型肝炎病毒X；肝細胞癌；入侵；轉移。

版權所有©2020 Editrice Gastroenterologica Italiana S.r.l.由Elsevier Ltd.出版。保留所有權利。