N末端有所作为：乙型肝炎病毒大表面蛋白N末端部分的基因型

StephenW

The N-Terminus Makes the Difference: Impact of Genotype-Specific Disparities in the N-Terminal Part of The Hepatitis B Virus Large Surface Protein on Morphogenesis of Viral and Subviral Particles
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August 23, 2020
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Cells. 2020 Aug 13;9(8):E1898. doi: 10.3390/cells9081898.

ABSTRACT

The N-terminus of the hepatitis B virus (HBV) large surface protein (LHB) differs with respect to genotypes. Compared to the amino terminus of genotype (Gt)D, in GtA, GtB and GtC, an additional identical 11 amino acids (aa) are found, while GtE and GtG share another similar 10 aa. Variants of GtB and GtC affecting this N-terminal part are associated with hepatoma formation. Deletion of these amino-terminal 11 aa in GtA reduces the amount of LHBs and changes subcellular accumulation (GtA-like pattern) to a dispersed distribution (GtD-like pattern). Vice versa, the fusion of the GtA-derived N-terminal 11 aa to GtD causes a GtA-like phenotype. However, insertion of the corresponding GtE-derived 10 aa to GtD has no effect. Deletion of these 11aa decreases filament size while neither the number of released viral genomes nor virion size and infectivity are affected. A negative regulatory element (aa 2-8) and a dominant positive regulatory element (aa 9-11) affecting the amount of LHBs were identified. The fusion of this motif to eGFP revealed that the effect on protein amount and subcellular distribution is not restricted to LHBs. These data identify a novel region in the N-terminus of LHBs affecting the amount and subcellular distribution of LHBs and identify release-promoting and -inhibiting aa residues within this motive.

PMID:32823751 | DOI:10.3390/cells9081898

StephenW

N末端有所作为：乙型肝炎病毒大表面蛋白N末端部分的基因型特异性差异对病毒和亚病毒颗粒形态的影响
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2020年8月23日
本文最初发表在这里

细胞。 2020年8月13日; 9（8）：E1898。 doi：10.3390 / cells9081898。

抽象

乙型肝炎病毒（HBV）大表面蛋白（LHB）的N端在基因型方面有所不同。与基因型（Gt）D的氨基末端相比，在GtA，GtB和GtC中发现了另外11个相同的氨基酸（aa），而GtE和GtG具有另一个相似的10个氨基酸。影响该N末端部分的GtB和GtC的变体与肝癌的形成有关。 GtA中这些氨基末端11aa的缺失减少了LHBs的数量，并将亚细胞积累（GtA样模式）改变为分散的分布（GtD样模式）。反之亦然，GtA衍生的N端11aa与GtD融合会导致GtA样表型。但是，将相应的源自GtE的10aa插入GtD无效。这些11aa的删除减少了细丝的大小，而释放的病毒基因组的数目，病毒体的大小和感染性均不受影响。确定了影响LHBs数量的负调控因子（aa 2-8）和显性正调控因子（aa 9-11）。该基序与eGFP的融合表明，对蛋白质数量和亚细胞分布的影响不仅限于LHBs。这些数据确定了LHBs N末端的一个新区域，该区域影响LHBs的数量和亚细胞分布，并确定了该动机内促进释放和抑制氨基酸残基。

PMID：32823751 | DOI：10.3390 / cells9081898