肝細胞癌中HBV突變與整合的相關性研究

StephenW

Biosci Rep

. 2020 Aug 14;BSR20201988.
doi: 10.1042/BSR20201988. Online ahead of print.
Studies on the correlation between mutation and integration of HBV in hepatocellular carcinoma
Xiaofang Cui  1 , Wei Wei  1 , Chao Wang  2 , Yanwei Qi  3 , Xiao Qin  1 , Lizhen Huang  4 , Weiyang Li  1
Affiliations
Affiliations

    1
    Jining Medical University, Jining, China.
    2
    Jining Hospital of Traditional Chinese Medicine, Jining, China.
    3
    Shenzhen Research Institute of City University of Hong Kong, Shenzhen, China.
    4
    South China University of Technology, Guangzhou, China.

    PMID: 32797164 DOI: 10.1042/BSR20201988

Abstract

It is well known that both the mutation and integration of the Hepatitis B virus (HBV) are of great significance in liver cancer, however, the relationship between mutation and integration is still unclear. In this study, sequencing data from 426 previously published samples were analyzed and 5374 specific HBV mutations in cancer tissues were discovered. By comparing integrated samples and non-integrated samples, we found that the integrated samples had higher sample single nucleotide variants (SNV) positive rates and SNV numbers, as well as higher sample frequency of SNV in the X region of the HBV genome. Samples with HBV integration in the telomerase reverse transcriptase (TERT) region showed higher SNV positive rates and numbers than samples without integration. Moreover, the SNVs (209[T>G] and 531[T>C; T>G]) were seen with higher frequency in samples with integration in the TERT region. Our study showed that the occurrence of viral integration events is closely related to the occurrence of SNV, and SNV in the X region should be more directly associated with viral integration. This study provides an initial exploration of the relationship between HBV mutation and integration to help improve our understanding of the relationship between viral integration and mutation.

Keywords: HBV; HBV integration; HCC; SNV; TERT.

Copyright 2020 The Author(s).

StephenW

生物科學代表

。 2020年8月14日； BSR20201988。
doi：10.1042 / BSR20201988。在線印刷。
肝細胞癌中HBV突變與整合的相關性研究
崔小芳1，魏薇1，王超2，閆偉奇3，小琴1，黃麗珍4，李維揚1
隸屬關係
隸屬關係

    1個
    濟寧醫科大學，中國濟寧
    2
    濟寧市濟寧市中醫院。
    3
    香港城市大學深圳研究所，中國深圳。
    4
    華南理工大學，中國廣州。

    PMID：32797164 DOI：10.1042 / BSR20201988

抽象

眾所周知，乙型肝炎病毒（HBV）的突變和整合在肝癌中均具有重要意義，但是，突變和整合之間的關係仍不清楚。在這項研究中，分析了來自426個先前發表的樣本的測序數據，並在癌症組織中發現了5374個特定的HBV突變。通過比較整合樣本和非整合樣本，我們發現整合樣本在HBV基因組的X區域中具有較高的樣本單核苷酸變異（SNV）陽性率和SNV數量，以及較高的SNV樣本頻率。端粒酶逆轉錄酶（TERT）區域中具有HBV整合的樣品比沒有整合的樣品顯示更高的SNV陽性率和數量。此外，在帶有TERT區域積分的樣本中，SNV（209 [T> G]和531 [T> C； T> G]）的出現頻率更高。我們的研究表明，病毒整合事件的發生與SNV的發生密切相關，X區域的SNV應該與病毒整合更直接相關。這項研究提供了對HBV突變與整合之間關係的初步探索，以幫助增進我們對病毒整合與突變之間關係的理解。

關鍵字：乙肝病毒； HBV整合； HCC； SNV； TERT。

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StephenW

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