细胞因子与乙型肝炎病毒及其抗原的关联

StephenW

Association of cytokines with hepatitis B virus and its antigen
Ming-Hui Li  1   2 , Qi-Qi Chen  2 , Lu Zhang  1 , Hui-Hui Lu  1 , Fang-Fang Sun  1 , Zhan Zeng  2 , Yao Lu  1 , Wei Yi  3 , Yao Xie  1   2
Affiliations
Affiliations

    1
    Department of Hepatology Division 2, Beijing Ditan Hospital, Capital Medical University, Beijing, 100015, China.
    2
    Department of Hepatology Division 2, Peking University Ditan Teaching Hospital, Beijing, Beijing, 100015, China.
    3
    Department of Gynecology and Obstetrics, Beijing Ditan Hospital, Capital Medical University, Beijing, 100015, China.

    PMID: 32662892 DOI: 10.1002/jmv.26301

Abstract

Objective: To investigate the characteristics of cytokines in patients with different HBV infection status and their correlation with HBV DNA, HBsAg and HBeAg levels.

Methods: Peripheral blood samples were collected from patients with chronic HBV infection in immune tolerance phase (IT), HBeAg-positive chronic hepatitis B (CHB) and acute hepatitis B (AHB) groups, and levels of cytokines were detected by Luminex technique, and analyzed by FLEXMAP 3D analyzer. The correlation between cytokines and HBV DNA load, HBsAg, HBeAg and ALT level in patients with chronic HBV infection was analyzed.

Results: In total 312 subjects (184 males, 128 females) were enrolled in the study. There were significant differences among IT, CHB, and AHB groups in Flt-3L value ((P=0.003; H=12.312), IFN-γ (P=0.001; H=11.723), IL-10 (P=0.001; H=18.736), IL-17A ((P= 0.001; H=12.735), and TGF-β1 ((P=0.001; Z=48.571). IFN-α2 levels in CHB group were significantly higher than those in IT and AHB groups (15.24 vs 35.78 pg/ml, P=0.000, Z=3.727; 13.88 vs. 35.78 pg/ml, P=0.024; Z=-2.258. In CHB group, the levels of HBsAg and ALT were positively correlated with the levels of IL-10 (r=0.173, P=0.006; r=0.176, P=0.006, respectively), while HBeAg level was positively correlated with the IFN-α2 level (r=0.153, P=0.016). In AHB group, the HBsAg level was positively correlated with Flt-3L, IFN-α2, IL-10 and IL-6 (r=0.402, P=0.023; r=0.436, P=0.016; r=0.524, P=0.002; r=0.405, P=0.022, respectively). HBeAg level was positively correlated with IFN-γ and IL-17A levels (r=0.400, P=0.023; r=0.373, P=0.036, respectively), and ALT level was positively correlated with IL-6 levels (r=0.367, P=0.039). In either AHB or CHB group, HBV DNA load was only related to TGF -β level (r=0.493, P=0.004; r=-0.218, P=0.009 respectively). The correlation between Flt-3L and HBsAg (F=7.422; P=0.007); IL-17, IL-6 and HBeAg (F=5.757, P=0.017; F=6.156, P=0.014) were statistically significant. There was significant correlation between TGF-β2 and HBV DNA (F=11.795; P=0.001), and between ALT and HBsAg, HBV DNA (F=26.089, P=0.000; F=4.724, P=0.031).

Conclusion: HBsAg, HBeAg, and HBV DNA were correlated with cytokines and ALT in patients with HBV infection. The level of IFN-α2 was significantly higher in patients with CHB. HBV DNA load was only correlated with the level of TGF-β in acute or CHB. This article is protected by copyright. All rights reserved.

Keywords: HBV DNA; HBeAg; HBsAg; chronic hepatitis B; cytokines.

This article is protected by copyright. All rights reserved.

StephenW

细胞因子与乙型肝炎病毒及其抗原的关联
李明慧1 2，陈启琦2，陆章1，陆慧辉1，孙芳芳1，詹增2，姚陆1，卫一3，姚X 1 2
隶属关系
隶属关系

    1个
    首都医科大学附属北京地坛医院肝病科2，北京100015
    2
    北京大学地坛教学医院第二医院肝科，北京100015
    3
    首都医科大学附属北京地坛医院妇产科，北京100015

    PMID：32662892 DOI：10.1002 / jmv.26301

抽象

目的：探讨不同HBV感染状态患者的细胞因子特征及其与HBV DNA，HBsAg和HBeAg水平的相关性。

方法：从免疫耐受期（IT），HBeAg阳性的慢性乙型肝炎（CHB）和急性乙型肝炎（AHB）组的慢性HBV感染患者中采集外周血，并通过Luminex技术检测细胞因子水平，并由FLEXMAP 3D分析仪进行分析。分析了慢性HBV感染患者细胞因子与HBV DNA负荷，HBsAg，HBeAg和ALT水平的相关性。

结果：总共312名受试者（184名男性，128名女性）被纳入研究。 IT，CHB和AHB组之间的Flt-3L值（（P = 0.003; H = 12.312），IFN-γ（P = 0.001; H = 11.723），IL-10（P = 0.001; H）有显着差异= 18.736），IL-17A（（P = 0.001; H = 12.735）和TGF-β1（（P = 0.001; Z = 48.571））。CHB组的IFN-α2水平显着高于IT和AHB组（15.24 vs. 35.78 pg / ml，P = 0.000，Z = 3.727； 13.88 vs. 35.78 pg / ml，P = 0.024； Z =-2.258。在CHB组中，HBsAg和ALT的水平与HBsAg的水平呈正相关。 IL-10（r = 0.173，P = 0.006； r = 0.176，P = 0.006），而HBeAg水平与IFN-α2水平呈正相关（r = 0.153，P = 0.016）。 HBsAg水平与Flt-3L，IFN-α2，IL-10和IL-6呈正相关（r = 0.402，P = 0.023; r = 0.436，P = 0.016; r = 0.524，P = 0.002; r = 0.405， P = 0.022）。HBeAg水平与IFN-γ和IL-17A水平呈正相关（r = 0.400，P = 0.023; r = 0.373，P = 0.036），而ALT水平与IL-A正相关6级（r = 0.367，P = 0.039）。在AHB或CHB组中，HBV DNA负荷仅与TGF-β水平相关（r = 0.493，P = 0.004； r = -0.218，P = 0.009）。 Flt-3L与HBsAg之间的相关性（F = 7.422; P = 0.007）; IL-17，IL-6和HBeAg（F = 5.757，P = 0.017; F = 6.156，P = 0.014）具有统计学意义。 TGF-β2与HBV DNA之间具有显着相关性（F = 11.795; P = 0.001），ALT与HBsAg与HBV DNA之间具有显着相关性（F = 26.089，P = 0.000; F = 4.724，P = 0.031）。

结论：HBs感染患者中HBsAg，HBeAg和HBV DNA与细胞因子和ALT相关。 CHB患者的IFN-α2水平明显更高。 HBV DNA负荷仅与急性或CHB患者的TGF-β水平相关。本文受版权保护。版权所有。

关键字：HBV DNA；乙肝抗原乙肝表面抗原慢性乙型肝炎细胞因子。

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