游泳作为慢性病毒性乙型肝炎患者不合并病毒而分离精子的

StephenW

Swim-up as a strategy for isolation of spermatozoa without viral incorporation in men with chronic hepatitis B: A pilot study
Thomas Condijts  1   2 , Liesl Bourdeaud'huy  1   2 , Kelly Tilleman  1 , Sylvie Lierman  1 , Chantal Dewinter  2 , Elizaveta Padalko  2
Affiliations
Affiliations

    1
    Department of Reproductive Medicine, Ghent University Hospital, Ghent, Belgium.
    2
    Department of Laboratory Medicine, Ghent University Hospital, Ghent, Belgium.

    PMID: 32658358 DOI: 10.1111/and.13732

Abstract

Hepatitis B virus (HBV) incorporates into spermatozoa which raises safety concerns about paternofetal transmission performing intracytoplasmatic sperm injection (ICSI) in men with chronic hepatitis B (cHB). HBV reduces sperm cell motility, assuming spermatozoa with highest motility are least HBV-incorporated. This study investigates an ICSI preparation technique (swim-up) to isolate most motile spermatozoa in order to select HBV-free spermatozoa. Semen and blood samples were collected from four patients with cHB. Spermatozoa were incubated in trajectories of gamete medium to create non-motile, motile/non-progressive and motile/progressive fractions. After DNA-extraction, HBV DNA loads were determined in every fraction. Participants (mean age 31) were HBsAg+(4/4), anti-HBc+(4/4) and HBV DNA+(2/4). They were treated (3/4) with entecavir(1/4) or tenofovir (2/4) and had no adverse sperm parameters(3/4). CRP-gene was detected in 95/96 sample fractions, proving successful DNA-extraction. HBV DNA was detected in none of the sample fractions, except for the motile, non-progressive fraction of one patient (HBeAg+, HBV DNA+). Since no HBV DNA was detected in progressive fractions, this study suggests swim-up a successful strategy to select HBV-free spermatozoa. Since all but one fraction was HBV DNA-negative, this study also suggests that patients with well-controlled disease have no HBV-contaminated sample fractions. This study encourages evaluation of guidelines restricting reproductive possibilities in men with cHB.

Keywords: HBV; infertility; spermatozoa; swim-up.

© 2020 Blackwell Verlag GmbH.

StephenW

游泳作为慢性病毒性乙型肝炎患者不合并病毒而分离精子的一种策略：一项初步研究
Thomas Condijts 1 2，Liesl Bourdeaud'huy 1 2，Kelly Tilleman 1，Sylvie Lierman 1，Chantal Dewinter 2，Elizaveta Padalko 2
隶属关系
隶属关系

    1个
    比利时根特根特大学医院生殖医学科。
    2
    比利时根特根特大学医院检验医学系。

    PMID：32658358 DOI：10.1111 / and.13732

抽象

乙型肝炎病毒（HBV）并入精子，这引起了对慢性乙型肝炎（cHB）男性进行胞浆内精子注射（ICSI）的pa行胎儿传播的安全性问题。假设具有最高运动能力的精子掺入最少的HBV，则HBV会降低精子细胞的运动能力。这项研究调查了一种ICSI制备技术（swim-up），以分离最能活动的精子，以选择不含HBV的精子。从四名cHB患者中收集精液和血液样本。将精子在配子培养基的轨迹中温育以产生非运动性，运动性/非进行性和运动性/进行性级分。 DNA提取后，确定每个部分的HBV DNA载量。参与者（平均年龄31岁）为HBsAg +（4/4），抗HBc +（4/4）和HBV DNA +（2/4）。他们用恩替卡韦（1/4）或替诺福韦（2/4）治疗（3/4），并且没有不利的精子参数（3/4）。在95/96样品级分中检测到CRP基因，证明成功提取了DNA。除了一名患者的活动性，非进行性部分（HBeAg +，HBV DNA +）外，所有样本均未检测到HBV DNA。由于未检测到逐步进行的HBV DNA提取，因此本研究提示选择无HBV精子的成功策略。由于除一小部分外，其余均为HBV DNA阴性，因此该研究还表明，疾病控制良好的患者没有被HBV污染的样品。这项研究鼓励评估限制cHB男性生殖能力的指南。

关键字：HBV；不孕症精子游上来。

©2020 Blackwell Verlag GmbH。