蒲公英和蒲公英酸对乙型肝炎病毒的体外抑制作用

StephenW

In Vitro Inhibition Effects of Hepatitis B Virus by Dandelion and Taraxasterol
Ying Yang  1 , Gaoxiang Ying  1 , Shanshan Wu  1 , Fengtian Wu  1 , Zhi Chen  1
Affiliations
Affiliation

    1
    State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Zhejiang, China.

    PMID: 32647534 PMCID: PMC7336670 DOI: 10.1186/s13027-020-00309-4

Abstract

Hepatitis B virus (HBV) causes hepatitis, which progresses to fatal liver diseases and remains a global health problem. Current treatments for chronic hepatitis B are unable to cure hepatitis. Thus, new antiviral drugs must be developed. In this study, the viral inhibition effects of dandelion and taraxasterol were assessed in HepG2.2.15 cell line. Taraxacum officinale F.H.Wigg. (compositae) with English name dandelion is used as a traditional herb for liver disorders and as a common antiviral agent. Taraxasterol is one of the active compounds of dandelion. The secretion of HBV DNA and HBV surface antigen (HBsAg) and HBeAg was detected using fluorescence quantitative PCR (qPCR) and ELISA, respectively. Intracellular HBsAg was detected by immunofluorescence. In order to demonstrate the potential mechanism of anti-viral activity, the expression levels of host factors polypyrimidine tract binding protein 1 (PTBP1) and sirtuin 1 (SIRT1) were detected with Western blotting and qPCR. Dandelion and taraxasterol effectively reduced the secretion of HBsAg, HBeAg and the HBV DNA in cell supernatants, and significantly reduced the intracellular HBsAg as indicated by immunofluorescence results. Taraxasterol may be one of the main effective components of dandelion. It significantly decreased the protein expression levels of PTBP1 and SIRT1. The present study revealed that dandelion and its component taraxasterol could inhibit HBV and may be a potential anti-HBV drug, whose potential targets were the host factors PTBP1 and SIRT1.

Keywords: Antiviral activity; Dandelion; Hepatitis B virus; Taraxasterol.

© The Author(s) 2020.

StephenW

蒲公英和蒲公英酸对乙型肝炎病毒的体外抑制作用
杨颖1，高翔颖1，吴珊珊1，吴奉天1，陈智1
隶属关系
联系

    1个
    浙江大学医学院附属第一医院传染病诊治国家重点实验室，国家传染病临床研究中心，传染病诊治合作创新中心。

    PMID：32647534 PMCID：PMC7336670 DOI：10.1186 / s13027-020-00309-4

抽象

乙型肝炎病毒（HBV）引起肝炎，并发展为致命性肝病，仍然是全球性的健康问题。目前对慢性乙型肝炎的治疗无法治愈肝炎。因此，必须开发新的抗病毒药物。在这项研究中，在HepG2.2.15细胞系中评估了蒲公英和蒲公英甾醇的病毒抑制作用。蒲公英（Taraxacum officinale F.H. Wigg） （菊科）英文名dandelion被用作肝病的传统草药和常见的抗病毒药。蒲公英甾醇是蒲公英的活性化合物之一。分别使用荧光定量PCR（qPCR）和ELISA检测HBV DNA和HBV表面抗原（HBsAg）和HBeAg的分泌。通过免疫荧光检测细胞内HBsAg。为了证明抗病毒活性的潜在机制，通过蛋白质印迹和qPCR检测了宿主因子聚嘧啶束结合蛋白1（PTBP1）和sirtuin 1（SIRT1）的表达水平。免疫荧光结果表明，蒲公英和蒲公英糖醇可有效减少细胞上清液中HBsAg，HBeAg和HBV DNA的分泌，并显着降低细胞内HBsAg。蒲公英甾醇可能是蒲公英的主要有效成分之一。它显着降低了PTBP1和SIRT1的蛋白表达水平。本研究表明，蒲公英及其成分酒石甾醇可以抑制HBV，可能是一种潜在的抗HBV药物，其潜在靶标是PTBP1和SIRT1宿主因子。

关键词：抗病毒活性；蒲公英乙型肝炎病毒;蒲公英甾醇。

©作者2020。

StephenW

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