使用培养的ELISPOT分析鉴定HBcAg特异性T细胞与慢性HBV感染中的

StephenW

Identification of the association between HBcAg-specific T cell and viral control in chronic HBV infection using a cultured ELISPOT assay.

Jul 6, 2020

Hepatitis B virus (HBV)-specific T cells play a critical role in determining the outcome of HBV infection. However, T cell response induced by predominant Ag in chronic infection is hardly detectable owing to the lack of a suitable assay. We herein established an optimized method to enumerate HBV-specific T cells and assessed the association between HBV surface Ag (HBsAg) and HBV DNA. Sixty chronic HBV infection patients were enrolled. HBV-specific T cells were expanded by using overlapping peptide pools covering the entire sequence of HBV genotypes B and C. IFN-γ-producing HBV-specific T cells were detected by a cultured enzyme-linked immunospot (ELISPOT) assay, ex vivo ELISPOT assay, or flow cytometry staining. The association between HBV-specific T cells and serum levels of HBsAg and HBV DNA were analyzed. Cultured ELISPOT assay had a higher sensitivity than ex vivo ELISPOT in the detection of HBV-specific T cells. Moreover, consistent results were acquired by flow cytometry analysi s and cultured ELISPOT assay, but the latter required only a limited number of cells for detection. Interestingly, HBV core peptide pool induced a robust HBV-specific T cell response in patients with lower levels of HBV DNA and HBsAg. Specifically, the frequency of HBV core Ag-specific IFN-γ spot-forming cells was inversely correlated with serum levels of HBV DNA and HBsAg. An optimized cultured ELISPOT assay reveals the association between HBV core Ag-induced T cell response and HBV control; this method may favor the investigation of HBV-specific T cell in chronic HBV infection.
©2020 Society for Leukocyte Biology.

StephenW

使用培养的ELISPOT分析鉴定HBcAg特异性T细胞与慢性HBV感染中的病毒控制之间的关联。

2020年7月6日

乙肝病毒（HBV）特异性T细胞在决定HBV感染的结局中起关键作用。然而，由于缺乏合适的检测方法，在慢性感染中主要由Ag诱导的T细胞反应很难检测到。我们在本文中建立了枚举HBV特异性T细胞的优化方法，并评估了HBV表面Ag（HBsAg）和HBV DNA之间的关联。招募了60名慢性HBV感染患者。通过使用覆盖整个HBV基因型B和C的整个序列的重叠肽库扩增HBV特异性T细胞。通过培养的酶联免疫斑点法（ELISPOT），离体ELISPOT检测出产生IFN-γ的HBV特异性T细胞。分析或流式细胞仪染色。分析了HBV特异性T细胞与血清HBsAg和HBV DNA水平之间的关系。培养的ELISPOT分析在检测HBV特异性T细胞方面比离体ELISPOT具有更高的灵敏度。此外，通过流式细胞仪分析和培养的ELISPOT分析获得了一致的结果，但是后者仅需要有限数量的细胞进行检测。有趣的是，HBV核心肽库在HBV DNA和HBsAg水平较低的患者中引起强烈的HBV特异性T细胞应答。具体而言，HBV核心Ag特异性IFN-γ点形成细胞的频率与血清HBV DNA和HBsAg呈负相关。优化的培养ELISPOT分析揭示了HBV核心银诱导的T细胞反应与HBV控制之间的联系。这种方法可能有助于研究慢性HBV感染中HBV特异性T细胞。
©2020年白细胞生物学学会。